Fast Room Temperature DNA Isolation Kits No RNase Added For Plants Genomic Dna Extraction

No RNase added fast room temperature Plant DNA Isolatoon kits for genomic dna extraction from plants

Description:

This Plant DNA Isolation kit uses a DNA-only column that can specifically bind DNA, Foregene protease and a unique buffer system, which greatly simplifies the purification of plant genomic DNA. High-quality genomic DNA can be can be obtained within 30 minutes, which avoid the degradation of genomic DNA.

The DNA-only silica gel membrane used in the spin column is Foregene's unique new material, which can effectively and specifically bind to DNA, and maximize the removal of RNA, impurity proteins, ions, polysaccharides, polyphenols and other organic compounds.

Specifications:

50 Preps, 100 Preps,250 Preps

Product components:

Product Information

Features&advantages:

-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.

-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;

-Simple: the genomic DNA purification operation can be completed in 30 minutes.

-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.

-Safety: no organic reagent is used.

-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Kit application:

It is suitable for extraction and purification of genomic DNA from fresh or frozen plant tissues.

Kits component information

- BUFFER PL1: Provides plant tissue cracking environments.

- Foregene Protease: Protein in the enzymatic plants in the environment of Buffer PL1.

- BUFFER PL2: Factory ForeGene Protease and provides a DNA upper column environment.

- BUFFER PW: Remove protein, RNA and other impurities in DNA.

- BUFFER WB: Remove residual salt ions in DNA.

- BUFFER EB: Elution of DNA on the purified column membrane.

- Genetological DNA in specific adsorption lysis.

DNA-Only Column characters

*: The minimum elution system of 100μl is a reasonable recommended volume given in consideration of DNA recovery rate and concentration. If in order to increase the yield of DNA, the volume of the eluate can be appropriately increased; if in order to increase the concentration of purified DNA, the volume of the eluate can be appropriately reduced on the premise of sacrificing a part of the DNA yield, such as using a 50 μl elution system, in order to obtain higher concentrations of DNA.

Genomic DNA extraction yield and purity

The amount of plant genomic DNA obtained by extraction is related to the source of the plant sample, the freshness of the sample, the storage condition, the moisture content and other factors. The Plant DNA Isolation Kit was used to process fresh leaf samples of plants from various sources. The amount and purity of genomic DNA obtained are shown in the following table:

Note: The data in this table is for reference only. In actual operation, due to factors such as storage conditions of the materials used, operating proficiency, etc., the data obtained may be slightly different from the data in this table.

Genomic DNA Fragment Size

The Plant DNA Isolation kit uses silica gel membrane column to separate and purify plant genomic DNA, and the size of the purified genomic DNA fragments are all around 23kb. As shown in the figure:

Note: (Be sure to read the notes carefully before using)

• The sample should avoid repeated freezing and thawing, otherwise the extracted DNA fragment will be small and the extracted quantity will be reduced.
• Do not use more than 100 mg of fresh plant leaves or tissue and 30mg of dried plant tissue, otherwise DNA yield and purity will be affected.
• Before use, carefully check whether there is precipitation in Buffer PL1, Buffer PL2 and Buffer PW. If there is precipitation, please dissolve it at 37℃ and mix it before use.
• Before using the kit, be sure to check whether anhydrous ethanol is added to Buffer WB as instructed. Before Buffer WB was used, 60mL, 120 ml and 300 ml of anhydrous ethanol (DE-06111) were added, respectively.
• Elution volume: Buffer EB should not be less than 100μl, otherwise DNA production will be affected.
• Do not add RNase to any Buffer.
• All centrifugation steps are performed in a table top centrifuge at room temperature (15-25℃).
• All experimental procedures were performed at room temperature (15-25℃).

Work Flow:

Diagram:

Storage and Shelf life:

The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.