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Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent

Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent

  • Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent
  • Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent
  • Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent
Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 50T, 100T, 250T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent
CAT NO.: DE-05111, DE-05112, DE-05113 Type: DNA-Only Column
Application: Extraction And Purification Of Genomic DNA From Fresh Or Frozen Anticoagulated Whole Blood Operation Temperature: Room Temperature
High Light:

Lab Reagent Blood DNA Midi Kit

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Molecular Biology Lab DNA Midi Kit

Molecular biology lab reagent Blood DNA Midi Kit lab DNA extraction reagent

 

 

Description

 

Blood DNA Midi Kit adopts new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulant blood samples in a short period of time, thus avoiding DNA degradation to the maximum degree and obtaining the maximum amount of genomic DNA.The rapid blood processing system and the simple centrifugal column operation greatly simplify the extraction of blood genome, making the genomic DNA of high quality and high purity can be obtained within 40 minutes.

The DNA-only silica gel membrane used in the centrifuge column of this kit is a new type of material unique to our company, which can adsorb DNA efficiently and specifically, and can remove RNA, impurities, proteins, ions and other organic compounds in cells to the maximum extent.The obtained DNA fragments are large, high purity, stable and reliable quality. The maximum carrying capacity of a centrifugal column is 80μg DNA.The obtained DNA can be used in molecular biology experiments such as enzyme digestion, PCR, Southern hybridization and library construction.

Blood DNA Midi Kit can process up to 1ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.

 

 

Kit conponents

 

Blood DNA Midi Kit
Kit contents DE-05121 DE-05122 DE-05123
50 T 100 T 250 T
Buffer BL1 25ml 50ml 125ml
Buffer BL2 25ml 50ml 125ml
Buffer DC 500ml 500ml x2 500ml x5
Buffer PW 25ml 50ml 125ml
Buffer WB1 15ml 30ml 75ml
Buffer EB 10ml 20ml 50ml
Foregene Protease Plus 2.5ml 5ml 12.5ml
DNA-Only Column 50 sets 100 sets 250 sets
IFU 1 piece 1 piece 1 piece
 

Features&advantages

 

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.

- Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.

- Safety: No organic reagent extraction is used.

- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Kit components

 

- Buffer DC: Lyses red blood cells in the blood.

- Buffer BL1: Provide sample enzymolysis environment.

- Foregene Protease Plus: Enzymatically hydrolyze blood samples in the environment of Buffer BL1.

- Buffer BL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.

- Buffer PW: Remove impurities such as protein and RNA in DNA.

- Buffer WB1: Remove residual salt ions in DNA.

- Buffer EB: elute the DNA on the purification column membrane.

- DNA-Only Column: Specifically adsorb genomic DNA in the lysate.

 

Genomic DNA extraction yield and purity

 

The extraction yield of blood genomic DNA is related to the tissue source, storage conditions, storage time, dosage and other factors. The purity of the obtained genomic DNA meets the routine molecular biology experiment operation, and OD260/280 is between 1.7-1.9.

 

Genomic DNA fragment size

 

The Blood DNA Midi kit uses silica membrane columns to isolate and purify genomic DNA from a variety of sample sources. The purified genomic DNA fragments size are all around 23 kb.

 

Storage and Shelf life

 

Blood DNA Midi Kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

 

Molecular Biology Lab Reagent Blood DNA Midi Kit Lab DNA Extraction Reagent 0

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

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Tel:86-028-86050301
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