Mouse Tail DNA Mini Isolation Kit Extract Genomic DNA From Mouse Tail Within 1 Hour

Mouse Tail DNA Mini Isolation Kit extract high-quality genomic DNA from mouse tail within 1 hour​

Product Descprition

Due to the structure and properties of the mouse tail itself, the extraction of mouse tail genomic DNA has always troubled researchers: one is that the processing time of the mouse tail is long and the genomic DNA is easily degraded, the other is difficult to handle and the purity of the extracted genomic DNA is low.

High DNA extraction yield and purity Mouse Tail Direct PCR Kit without prior DNA purification uses a DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can digest mouse tail samples within 45 minutes, thereby minimizing the degradation of genomic DNA and shortening sample processing time. The kit can extract 10-20μg of high-quality, high-purity genomic DNA from 0.5-1cm juvenile or adult mouse tails within 60-90min.

The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in the cell. The extracted genomic DNA fragment is large, high in purity, stable and reliable in quality, and the size of the DNA fragment is stable at about 23kb.

The kit can also be used to extract other mouse tissues outside the mouse tail, including mouse ears, mouse muscle, mouse liver and other tissues, so that one kit can be used for multiple purposes and meet the needs of users for different experiments.

Kit Content:

*: Buffer TL2 and Buffer PW contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.

Features

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.

- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: No organic reagent extraction is required.

- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Genomic DNA Application

The genomic DNA purified by the Mouse Tail DNA Mini Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.

Kit components

- Buffer TL1: Provide mouse tail enzymatic hydrolysis environment.

- Foregene Protease Plus: Enzymatically digest tissue samples in the environment of Buffer TL1.

- Buffer TL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.

- Buffer PW: Remove impurities such as protein and RNA in DNA.

- Buffer WB: Remove residual salt ions in DNA.

- Buffer EB: Elute the DNA on the purification column membrane.

- DNA-only Column: Specifically adsorb genomic DNA in the lysate.

Genomic DNA extraction yield and purity

The yield of mouse tail genomic DNA is related to sample size, storage conditions, degree of enzymolysis and operation. Mouse tail DNA Mini kit provides an effective and rapid method to obtain mouse tail genomic DNA. The obtained genomic DNA has high purity and low degradation, and OD₂₆₀/₂₈₀=1.7-1.9.

Genomic DNA fragment size

Mouse Tail DNA Mini Kit uses a silica membrane column to isolate and purify mouse tail genomic DNA. The purified genomic DNA fragments size are all around 23 kb. As shown in the figure:

Storage conditions

- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

- Foregene Protease Plus solution has a unique formula, it is active when stored at room temperature for a long time (3 months); stored at 4°C, the activity and stability will be better, so it is recommended to store it at 4°C, remember not to keep it at -20°C.