Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 
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| Place of Origin: | China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | DE-05011/05012/05013 |
| Minimum Order Quantity: | 1 Kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 50T,100T,250T |
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| Type: | Spin Column | Applicable Samples: | Extraction And Purification Of Genomic DNA |
| Operation Temperature: | Room Temperature | Product Name: | Lab Nucleic Acid Purification Kit Extraction Kit DNA Isolation Kit For Animal Tissue |
| High Light: | Lab Nucleic Acid Purification Kit,250T DNA Isolation Kit |
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Lab Nucleic Acid Purification Kit Extraction Kit DNA isolation kit For Animal Tissue
Product Descprition:
This kit uses a DNA-only column that can specifically bind DNA, Foregene protease and a unique buffer system. High-quality genomic DNA can be extracted from various cultured cells and animal tissues within 30 to 50 minutes.
The DNA-only silica gel membrane used in the spin column is Foregene's unique new material, which can effectively and specifically bind to DNA, and maximize the removal of RNA, impurity proteins, ions and other organic compounds in cells. 5-80μg high-quality genomic DNA can be purified from 10-50mg tissue.
Product components:
| Animal Tissue DNA Isolation Kit | |||
| Kit content | DE-05011 | DE-05012 | DE-05013 |
| 50T | 100T | 250T | |
| Buffer L1 | 20ml | 40ml | 100ml |
| Buffer L2* | 20ml | 40ml | 100ml |
| Buffer PW* | 25ml | 50ml | 125ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease | 1.25ml | 2.5ml | 6.5ml |
| DNA-Only Column | 50 | 100 | 250 |
| Manual | 1 | 1 | 1 |
*: Buffer L2 and Buffer PW contain irritating desalted salt. Please wear gloves and take relevant protective measures when operating.
Features&advantages:
-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;
-Simple: the genomic DNA purification operation can be completed in 50 minutes.
-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
-Safety: no organic reagent is used.
-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit Component Information
- Buffer L1: Provides the enzymatic hydrolysis environment for animal tissue samples.
- Foregene Protease: Enzyme digestion of tissue samples in the environment of Buffer L1.
- Buffer L2: Inactivates Foregene Protease and provides a DNA-on-column environment.
- Buffer PW: Remove impurities such as protein and RNA from DNA.
- Buffer WB: removes residual salt ions from DNA.
- Buffer EB: Elute the DNA on the purification column membrane.
- DNA-only Column: Specific adsorption of genomic DNA in the lysate.
Genomic DNA Fragment Size
The Animal Tissue DNA Isolation Kit uses a silica membrane column to separate and purify genomic DNA from various sample sources, and the purified genomic DNA fragments are all around 23kb in size. As shown in the figure:
Product information
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
30-50min (24 samples) |
| Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 100-200μl | Bacterial liquid processing capacity | 10-50mg |
Storage and Shelf life:
- The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37℃ water bath for 10 minutes to dissolve the precipitate, and mix it before use.
- Foregene Protease solution has a unique formula and is active for a long time (3 months) at room temperature; its activity and stability will be better when stored at 4℃, so it is recommended to store it at 4℃, remember not to store it at -20℃ save.
Contact Person: Maggie
Tel: +8615281067355
