Place of Origin: | China |
Brand Name: | FOREGNE |
Certification: | ISO CE |
Model Number: | DE-01001, DE-01002, DE-01003 |
Minimum Order Quantity: | 1 kit |
---|---|
Price: | Negotiable |
Packaging Details: | Packing with safe trans carton |
Delivery Time: | 5-8 days |
Payment Terms: | L/C, T/T, Western Union |
Supply Ability: | 100000 kits per month |
Product Name: | Molecular Biology Lab Reagent General Plasmid Mini Kit Large DNA Isolation Kit | OEM: | Accept |
---|---|---|---|
Packing: | 50T, 100T, 250T | Application: | Transformation, Restriction Enzyme Digestion, PCR, Library Construction, Transfection,sequencing |
Feature: | No RNase Contamination,safe,fast,high Quality | Brand: | FOREGENE |
High Light: | Lab Reagent General Plasmid Mini Kit,Molecular Biology Lab DNA Isolation Kit |
Molecular biology lab reagent General Plasmid Mini Kit large DNA isolation kit
Description:
The FOREGENE’S nucleic acid extraction and purification series products can be extracted and purified to high-quality nucleic acids from a variety of sources and complex component samples. The DNA extraction series products are based on DNA-Only silica membrane purification columns and unique formula reagents that can purify high-quality DNA from a variety of samples.
The General Plasmid Mini Kit uses DNA-Only purification column technology and an efficient SDS lysis formula to obtain high-quality plasmid DNA from bacteria within 20 minutes. The DNA-Only silica gel membrane used in the spin column is a unique new material of FOREGENE, which can adsorb DNA efficiently and specifically,and remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum binding capacity of plasmid DNA of DNA-Only purification column is 60μg DNA. The amount of plasmid DNA actually extracted is related to the volume of the bacterial solution, the number of plasmid copies (high copy, low copy), strains, culture conditions and other factors.
Kit components:
General Plasmid Mini Kit | |||
Kit content | DE-01001 | DE-01002 | DE-01003 |
50T | 100T | 250T | |
Buffer S1 | 10ml | 20ml | 50ml |
Buffer S2 | 10ml | 20ml | 50ml |
Buffer S3 | 25ml | 50ml | 125ml |
Buffer PW | 25ml | 50ml | 125ml |
Buffer WB2 | 15ml | 30ml | 75ml |
Buffer EB | 10ml | 20ml | 50ml |
DNA-Only Column | 50 | 100 | 250 |
Manual | 1 | 1 | 1 |
Features&advantages:
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA in the plasmid DNA without adding RNase during the experiment, avoiding the contamination of the laboratory by exogenous RNase.
-Fast speed: Plasmid DNA extraction can be completed within 20 minutes.
-Safe: No organic reagent extraction is required.
-High quality: The plasmid obtained by extraction has no RNA, no RNase and extremely low ion content, which can meet the requirements of various experiments.
Product Quality Control:
In accordance with FOREGENE's Total Quality Management System, each batch of General plasmid mini kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Kit components information:
- Buffer S1:Provides bacterial lysis environment.
- Buffer S2:Lyse bacteria, denature protein and DNA.
- Buffer S3:Precipitates protein and genomic DNA, and provides a column environment for plasmid DNA.
- Buffer PW:: Remove impurities such as protein and RNA from plasmid DNA.
- Buffer WB2:Remove residual salt ions from plasmid DNA.
- Buffer EB:Elute the plasmid DNA on the purification column membrane.
- DNA-Only Column:Specific adsorption of plasmid DNA in the lysate.
Product information:
Model | Spin-column type | Purification components | Foregene Spin column, reagent |
Flux | 1-24 samples | Preparation time |
20min (24 samples) |
Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
Purification column DNA load carrying capacity | 60μg | Liquid volume of spin column | 800μl |
Elution volume | 50-200μl | Bacterial liquid processing capacity | 1-5ml |
Storage and Shelf life:
This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.
Contact Person: Maggie
Tel: +8615281067355