Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | FOREGNE |
| Certification: | ISO CE |
| Model Number: | DE-05611/05612/05613/05614/05615 |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | Packing with safe trans carton |
| Delivery Time: | 5-8 days |
| Payment Terms: | L/C, T/T, Western Union |
| Supply Ability: | 100000 kits per month |
| Product Name: | No RNase Contamination Lab Reagent Water DNA Isolation Kits For Genomic DNA Purification | OEM: | Accept |
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| Packing: | 50T, 100T, 250T | Application: | Purification Of The Genomic DNA |
| Feature: | Convenient，Fast，Safe，High Purity | Brand: | FOREGENE |
| High Light: | OEM Genomic DNA Purification Kits,Lab Reagent Water DNA Isolation Kits,DNA Isolation Kits No RNase Contamination |
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No RNase contamination lab reagent Water DNA Isolation Kits for genomic DNA purification
Description:
Water DNA Isolation Kit provides a quick and easy method for extracting genomic DNA from water samples of various sources. There are a large number of microorganisms in water samples, and these microorganisms are widely used as important ecological indicators. Extracting high-purity genomic DNA from water bodies is a very important method for water environmental value detection.
Water samples contain a large number of inhibitors such as humic acid, metal ions, etc., even if these substances are present in the purified DNA in small amounts, they will affect downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purifying water genomic DNA is to effectively remove inhibitors in water. Adopting our company's unique DNA-only silica membrane spin column and solution formula, matched with Foregene Protease, can effectively remove various inhibitors in water, without organic solvent extraction or ethanol and isopropanol precipitation, and DNA extraction operation can be completed in water samples within 40 minutes.
Kit components:
| Water DNA Isolation Kit | |||||
| Kit contents | DE-05611 | DE-05612 | DE-05613 | DE-05614 | DE-05615 |
| 5 T | 25 T | 50 T | 100 T | 250 T | |
| Buffer SG1 | 3.5ml | 18ml | 35ml | 70ml | 180ml |
| Buffer SG2 | 200μl | 1ml | 2ml | 4ml | 10ml |
| Buffer SG3 | 3.5ml | 18ml | 35ml | 70ml | 180ml |
| Buffer SG4 | 120μl | 600μl | 1.2ml | 2.4ml | 6ml |
| Buffer PW | 3ml | 15ml | 30ml | 60ml | 150ml |
| Buffer WB | 2.5ml | 12.5ml | 25ml | 50ml | 125ml |
| Buffer EB | 1.5ml | 6ml | 15ml | 60ml | 120ml |
| Buffer TE | 6ml | 30ml | 60ml | 120ml | 300ml |
| Foregene Protease | 180μl | 1ml | 1.8ml | 1.8ml×2 | 10ml |
|
Lysozyme |
50mg |
50mg×3 |
250mg |
500mg |
500mg×2 250mg×1 |
| DNA-Only Column | 5 sets | 25 sets | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece | 1 piece | 1 piece |
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time | 40min(24 samples) |
| Centrifuge | Table centrifuge | Tissue enzymolysis separation | centrifugal separation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 50-200μl | Water sample processing volume | 10ml-30L |
Kit Application
It is suitable for the purification of the genomic DNA of the following samples: aquaculture pond water, pond water, tap water, lake water, river water, lotus pond water and other samples.
Applications of genomic DNA
The genomic DNA purified by the Water DNA Isolation Kit is of high purity and can be used for routine molecular biology operations, such as: enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Quality Control
In accordance with FOREGEN's Total Quality Management System (FOREGENE's Total Quality Management System), each batch of water genomic DNA extraction kits are strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits .
Features&advantages:
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: The operation is simple, and the water genomic DNA extraction operation can be completed within 40 minutes.
- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
- Safety: no need organic reagent extraction.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments
Genomic DNA extraction yield and purity
The amount of genomic DNA extracted from the water body is related to factors such as the source of the water body sample, turbidity, and microbial content. Process water samples from various sources according to the Water DNA Isolation Kit, and the amount and purity of genomic DNA obtained are shown in the following table:
| Source of water | The sample amount(ml) | DNA output(μg) | OD260/280 | OD260/230 |
| River water | 400 | 1-2 | 1.7-1.9 | 1.7-1.9 |
| Pond water | 400 | 5-7 | 1.7-1.9 | 1.7-1.9 |
| Aquaculture pond water | 20 | 1-2 | 1.7-1.9 | 1.8-1.9 |
| Tap water | 4000 | 0.05-0.1 | ≈1.8 | 1.9-2.0 |
Note: The data in this table is for reference only. In actual operation, due to the storage conditions of the materials used, operating proficiency and other factors, the data obtained will be slightly different from the data in this table.
Genomic DNA fragment size:
The water body genomic DNA extraction kit uses silica gel membrane columns to separate and purify water body genomic DNA, and the purified genomic DNA fragments are all around 23 kb in size. As shown in the figure:
Storage conditions:
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
- Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); when stored at 4℃, its activity and stability will be better, so it is recommended to store it at 4℃, remember not to store it at -20℃.
- Dry powder Lysozyme is stored at -20°C; the prepared Lysozyme solution is divided into small portions and stored at -20°C.
Contact Person: Maggie
Tel: +8615281067355
