High Purity Lab Reagent General Plasmid Mini DNA Isolation Kit Purification Column

Fast and high purity lab reagent General Plasmid Mini DNA Isolation Kit DNA Isolation kits purification column

Description:

Foregene fast and high purity lab reagent General plasmid mini Kit adopts DNA-only purification column technology and efficient SDS lysis method to obtain high-quality Plasmid DNA from bacteria Based on the company's unique DNA-only purification column and unique formula reagent, can maximize the removal of RNA, without adding RNA enzyme, can remove RNA effect, so that the laboratory from RNA enzyme pollution but also can efficiently remove impurities in protein ions and other organic compounds in bacteria The actual plasmid DNA yield and purity were related to the host bacteria species and culture conditions bacterial lysis and plasmid copy number (high or low copy) and other factors.

This kit is suitable for plasmid DNA extraction of Gram-negative bacteria. The obtained plasmid DNA has high purity, no RNase and very low ion content, which can meet the requirements of various conventional experiments, such as invertase PCR library construction and sequencing (ddH₂O eluting plasmid DNA is recommended).

Kit components:

Applications of Plasmid DNA:

The plasmid DNA purified by the General Plasmid Mini Kit is of high purity and can be used for routine molecular biology operations, such as: transformation, enzyme digestion, PCR, library construction, transfection (passage cell transfection), and sequencing (it is recommended to use ddH₂O to elute plasmid DNA ).

Features&advantages:

-Remove RNA without adding RNase

-Convenient—the centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.

-Fast—the operation can be completed in 20 minutes

-Safe—no organic reagent used

-High purity—OD260/280≈1.7-1.9

Plasmid DNA extraction rate:

Detection of DNA concentration and purity:

- The quality of the plasmid DNA obtained depends on a variety of factors during the operation. The concentration and purity of DNA can be determined by agarose gel electrophoresis and ultraviolet spectrophotometer.

- DNA with an OD₂₆₀ value of 1 corresponds to about 50μg/ml of double-stranded DNA.

- DNA OD₂₆₀/₂₈₀ not less than 1.7 If deionized water is used for elution, ensure that the pH is in the range of 7.0-8.5. A pH below 7.0 will reduce elution efficiency and the OD₂₆₀/₂₈₀ ratio will be low.

Diagram:

Precautions

- The amount of bacterial solution should not be too much. OD₆₀₀=2.0-3.0 is recommended, and the amount should not exceed 5ml, otherwise the yield and purity of extracted plasmid DNA will be affected.

- Before use, carefully check whether there is precipitation in Buffer S2 and Buffer S3. If there is precipitation, please dissolve it in 37℃ and mixed before use.

- Before using the kit, be sure to check whether anhydrous ethanol has been added to Buffer WB2.

- Elution volume: should not be less than 50μl, otherwise it will affect plasmid DNA yield.

- All the experimental steps were carried out at room temperature (15-25℃), including centrifugation steps by bench centrifuge at room temperature.

Prevent cross-contamination between samples:

To prevent cross-contamination between samples during sampling and purification, the following measures can be taken:
- During the sampling process, it should be ensured that each individual sample is used with non-polluting sampling equipment and disposable centrifuge tubes.
- When aspirating the sample or solution, be careful and gentle to avoid splashing the solution.
- After the centrifuge tube is shaken, it should be centrifuged briefly, and the cap should be opened after the liquid at the mouth of the tube is removed.
- Gloves should be worn during the entire operation. If gloves come into contact with samples and their solutions, they should be replaced immediately.

Storage and Shelf life:

This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.