Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | ForeQuant |
| Certification: | ISO CE |
| Model Number: | F4 |
| Minimum Order Quantity: | 1 set |
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| Price: | Negotiable |
| Packaging Details: | Packing with safe trans carton |
| Delivery Time: | 5-8 days |
| Payment Terms: | L/C, D/P, T/T |
| Supply Ability: | 1000 sets per year |
| Product Name: | Real-Time PCR System F4 QPCR Machine Polymerase Chain Reaction Thermal Cycler | Block Capacity: | 96 Wells |
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| Sample Volume: | 10-50 Ul | Temperature Control Mode: | Temperature Control Module |
| Heat Cycle: | Peltier Semiconductor | Temperature Setting Range: | 4-100 ℃ |
| High Light: | 96 wells RT qPCR Machine,96 wells DNA PCR Thermal Cycler,Peltier semiconductor DNA PCR Thermal Cycler |
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Real Time PCR F4 RT qPCR Machine Lab Equipment DNA PCR Thermal Cycler
Descripstion:
ForeQuant F4 and ForeQuant F6 Real-Time PCR System are used to run real-time fluorescence PCR experiments (Real-time PCR) and to analyze the experimental data. the instruments are operated in the laboratory, in conjunction with the corresponding reagents, for rapid and accurate quantitative and qualitative detection of target nucleic acids from human samples (e.g., blood, oral swabs, nasopharyngeal swabs, body fluids, etc.) or other analytes in the patient's body, or for melting curves, genotype analysis.
Features:
- Innovative optical detection system-higher sensitivity.
- Time-resolved signal acquisition technology-less optical path crosstalk.
- The unique hollow is the temperature control module-more stable and fast thermal cycle control.
- Humanized and fully functional software-more comprehensive application, easier operation.
Schematic diagram of temperature control module
Schematic illustration of optical scanning patterns
Conventional Parameters:
| Instrument specifications |
Size:520 mm(L) × 350 mm (W) × 370 mm (H)； Weight:25 kg |
| Packing specifications |
Size:710 mm(L) × 540 mm (W) × 580 mm (H)； Gross Weight:36 kg |
| Power supply specification |
power supply voltage:AC 230 V Frequency:50 Hz； Power:750 w |
| Communication specifications | USB ports |
| Use ambient |
Temperature:10℃ ~ 30℃； Humidity:≤85% RH no condensation； Atmospheric pressure:85.0 kPa ~ 106.0 kPa； Altitude:< 2000m |
| Storage and transportation environment |
Temperature:-20℃ ~ 55℃,the instrument packaging in the transport box； Humidity:≤85% RH |
| Running noise | Maximum noise when running instrument does not exceed 60dB (A) |
Instrument Technical Parameters:
| Thermal parameters | ||
| Heat cycle |
Peltier semiconductor |
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| Temperature control mode |
Temperature control module |
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| Maximum ramp rate | 3.6 ℃/s | |
| Temperature accuracy | ± 0.2 °C | |
| Temperature uniformity | ± 0.2 ℃ | |
| Optical parameters | ||
| Excitation source | Single color LED | |
| Detection device | High sensitivity MPPC(silicon photomultiplier tube) | |
| Fluorescent channel number | 6 channels(ForeQuant F6) | |
| Applicable to fluorescent | Channel 1 | FAM/SYBR Green etc |
| Channel 2 | VIC/JOE/HEX/TET etc | |
| Channel 3 | ROX/Texas Red etc | |
| Channel 4 | NED/TAMRA/Cy3 etc | |
| Channel 5 | Cy5 etc | |
| Channel 6 | FAM/SYBR Green etc | |
| Detection parameters | ||
| Sample size | 96 wells | |
| Sample volume | 10-50 μl | |
| Repeatability of fluorescence intensity detection | CV ≤3% | |
| Detection of fluorescence intensity | CV ≤5% | |
| Sample repeatability | CV ≤ 3% | |
| Linear fluorescence intensity detection | A linear regression coefficient │r│≥0.990 in 5 concentration gradients | |
| Sample linear correlation | A linear regression coefficient│r│≥0.980 in 5 concentration gradients | |
The so-called real-time Q-PCR technology refers to the method of adding fluorescent genes to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally quantitatively analyzing the unknown template through the standard curve. In the development of real-time technology, two important discoveries played a key role: (1) in the early 1990s, the discovery of the 5' exonuclease activity of Taq DNA polymerase, which can degrade specific fluorescent probes; needle, thus making indirect detection of PCR products possible. (2) The use of fluorescent double-labeled probes enables real-time monitoring of the entire reaction process in a closed reaction tube. The combination of these two findings and the commercial development of the corresponding instruments and reagents has led to the use of real-time Q-PCR methods in research work.
Storage and transportation environment:
Temperature: -20℃ ~ 55℃,the instrument packaging in the transport box；
Humidity: ≤85% RH
Contact Person: Foregene
Tel: +8613880676215
