Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | No RNase Contamination Lab Molecular Biology Reagent Buccal Swab/FTA Card DNA Isolation Kits |
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| CAT NO.: | DE-05811, DE-05812, DE-05813 | Type: | DNA-Only Column |
| Application: | Purification Of Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | Buccal Swab Genomic DNA Isolation Kits,FTA Card Genomic DNA Isolation Kits |
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Fast room temperature Buccal Swab/FTA card DNA Isolation kits for gemomic dna extraction
Description
Buccal Swab FTA Card DNA Isolation Kit provides an efficient and rapid method to obtain high-concentration genomic DNA from buccal swabs and FTA Card (blood spots). Using our company’s unique DNA-only silica membrane spin column and formula, combined with Foregene Protease, high-concentration, high-quality genomic DNA can be extracted in 80 minutes. The specially designed small purification column binds genomic DNA, and the DNA can be eluted with a small amount (15μl) elution system to increase the concentration of the obtained genomic DNA, which is convenient for downstream detection or experiment. The kit can process one or more samples at a time, and the purification process does not require extraction of organic substances such as phenol, chloroform, and time-consuming isopropanol or ethanol precipitation, and the operation is simple and time-saving.
The DNA-only silica gel membrane used in the spin column of this kit is a unique new material of the company, which efficiently and specifically adsorbs DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The obtained DNA fragments are large, with high purity, stable and reliable quality, and the maximum carrying capacity of a spin column is 80 μg DNA. The obtained DNA can be used for molecular biology experiments such as restriction digestion, PCR, Southern hybridization, library construction and so on.
Kit conponents
| Buccal Swab/FTA Card DNA Isolation Kit | |||
| Kit contents | DE-05811 | DE-05812 | DE-05813 |
| 50 T | 100 T | 250 T | |
| Buffer ST1 | 18ml | 36ml | 90ml |
| Buffer ST2 | 18ml | 36ml | 90ml |
| Linear Acrylamide | 250μl | 500μl | 1.25ml |
| Buffer PW | 25ml | 50ml | 125ml |
| Buffer WB | 25ml | 50ml | 125ml |
| Buffer EB | 10ml | 20ml | 50ml |
| Foregene Protease | 1.25ml | 2.5ml | 5ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
Product INFO
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
80min (24 samples) |
| Centrifuge | Table centrifuge | Tissue hydrolysate separation | centrifugal separation |
| Purification column DNA load carrying capacity | 30μg | Liquid volume of spin column | 800μl |
| Elution volume | 15-30μl | Sample processing volume volume |
1 buccal swab 1-3 slices of blood with a diameter of 3mm |
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease has higher activity than similar proteases, digests samples quickly; simple operation.
- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
- Safety: No organic reagent extraction is required.
- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
- Micro-elution system: It can increase the concentration of genomic DNA, which is convenient for downstream detection or experiment.
Genomic DNA Application
The genomic DNA purified by the Buccal Swab/FTA Card DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Quality Control
According to FOREGENE's Total Quality Management System , each batch of Buccal Swab/FTA card DNA isolation kit is strictly tested for multiple times to ensure the reliability and stability of the quality of each batch of kits.
DNA-Only Column Characters
| Maximum binding capacity | 30μg |
| Maximum loading volume | 800μl |
| Longset DNA fragment | 23kb |
| Minimum elution volume* | 15μl |
| Selection of samples | Buccal Swab,blood stains |
| Maximum amount of starting material* | 1 buccal swab, 1-3 blood stains |
*: The minimum elution system of 15μl is a reasonable recommended volume considering DNA recovery and concentration. If you want to increase the yield of DNA, you can appropriately increase the volume of the eluate; if you want to increase the concentration of purified DNA, at the expense of part of the DNA yield, appropriately reduce the volume of the eluate, in order to obtain a higher concentration of DNA.
Genomic DNA extraction yield and purity
The yield of genomic DNA in a small sample is related to the source of the sample, storage conditions, degree of enzymatic hydrolysis, and operation. The Buccal Swab/FTA Card DNA isolation Kit provides an effective method to obtain genomic DNA from a small sample, generally a buccal swab 0.5-3μg of genomic DNA can be obtained (related to the number of cells contained in the buccal swab); a blood spot with a diameter of 3mm can obtain 0.2-0.5μg of genomic DNA. The obtained genomic DNA has high purity, and OD260/280≈1.7-1.9.
Genomic DNA fragment size
The Buccal Swab/FTA Card DNA isolation Kit uses a silica membrane column to isolate and purify genomic DNA from a variety of sample sources. The purified genomic DNA fragments size are all around 23 kb. See below:
Extract 1 buccal swab and 3 genomic DNA with a diameter of 3mm blood stains. Two replicates of each sample are eluted according to 30μl Buffer EB, 5μl of gene DNA and 100bp DNA Ladder are coagulated in 1% agarose Gel electrophoresis.
Notes: (Please read the notes carefully before using the kit)
◆ One buccal swab is sufficient for each purification system. For multiple buccal swabs, please use multiple purification columns for purification.
◆ For single purification, it is recommended to use 1-3 FTA Cards with a diameter of about 3mm for experiments. If the blood card storage quality is not good, increase the usage amount.
◆ Before use, carefully check whether there is any precipitation in Buffer ST1, Buffer ST2 and Buffer PW. If there is precipitation, please dissolve it at 37°C and mix well before use.
◆ Before using the kit, be sure to check whether Buffer WB has been added with absolute ethanol according to the instructions. Add 60ml of absolute ethanol (DE-05811), 120ml of absolute ethanol (DE-05812), and 300ml of absolute ethanol (DE-05813) to Buffer WB before use.
◆ During the lysis of the sample, the sample should always be immersed in the lysis buffer. If the sample adheres to the cap and inner wall of the tube, it can be processed by short centrifugation.
◆Elution volume: The use of a micro-elution system will increase the DNA concentration, but the Buffer EB should not be less than 15μl, otherwise it will affect the DNA yield.
◆ Remember not to add RNase to any buffer.
◆ All centrifugation steps are centrifugation at room temperature (15-25°C) using a benchtop centrifuge.
◆ All experimental steps are carried out at room temperature (15-25℃)
Preparation before operation
Please read the instructions carefully before using this kit. The oral swab/blood card genomic DNA extraction kit is simple, convenient and fast to operate, and the instructions provide complete information and correct usage of the entire kit. Please prepare necessary experimental materials and equipment before use.
Storage and Shelf life
1. Buccal Swab FTA Card DNA Isolation Kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
2. Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
