Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | Foregene |
| Certification: | CE ISO |
| Model Number: | IM-02011/IM-02012/IM-02013 |
| Minimum Order Quantity: | 1ml |
|---|---|
| Price: | USD0.005/unit |
| Packaging Details: | ice pack box |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/P, T/T |
| Supply Ability: | 200L per month |
| Factory: | Foregene | Product Name: | Foreasy M-MLV Reverse Transcriptase |
|---|---|---|---|
| Lead Time: | 12 Days | Specifications: | 200 KU (1 ML),2000 KU (10 ML),5000 KU (25 ML) |
| Storage: | -20±5℃ | Shelf Life: | 2 Years |
| Package: | Box | ||
| High Light: | M-MLV Reverse Transcriptase,PCR Kits M-MLV Reverse Transcriptase |
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Foreasy M-MLV Reverse Transcriptase for cDNA synthesis primer extension PCR kits used with DNA Polymerase
Product Introduction
Foreasy M-MLV Reverse Transcriptase is a new reverse transcriptase expressed in E. coli engineered bacteria using genetic recombination technology. It is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. It has no RNase H activity, strong stability, strong RNA affinity, and high detection sensitivity.
Product components:
| Component | IM-02011 | IM-02012 | IM-02013 |
|
Foreasy Reverse Transcriptase (200 U/μL) |
200 KU (1 mL) | 2000 KU (10 mL) | 5000 KU (25 mL) |
|
5× Foreasy RT Buffer |
1 mL ×5 | 10 mL ×5 | 10 mL ×12 |
Storage
Store at -20±5℃ for 2 years or at -80℃ for long-term storage.
Application
Synthesis of the first strand of cDNA for RT-PCR and real-time RT-PCR
cDNA synthesis for cloning and expression.
Generation of labeled cDNA probes for microarrays.
Primer extension RNA analysis
Unit definition
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of [ 3H] dTTP in 10 min at 37°C using poly(A)oligo(dT)25 as template-primer.
Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 µL for 10 min at 37°C.
Contact Person: Maggie
Tel: +8615281067355
