Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 200T, 500T, 2000T |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment |
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| Function: | Extraction | Type: | Whole Blood Direct PCR |
| Application: | EDTA Anticoagulated Whole Blood | Operation Temperature: | -20℃ |
| High Light: | EDTA Blood Direct PCR Kits,ISO Extraction Direct PCR Kits |
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Strong amplification PCR system Blood SuperDirect PCR Kits With EDTA no pretreatment
Description
Blood SuperDirectTM PCR Kit-EDTA uses a unique PCR buffer system, which allows direct PCR with whole blood samples as a template without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.
2× SuperEasyTM Mix-EDTA contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.
Kit conponents
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Blood SuperDirectTM PCR Kit-EDTA EDTA Anticoagulant whole blood direct PCR kit |
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Kit composition |
TP-0411T | TP-04111 | TP-04112 | TP-04113 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix-EDTA | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
- There is no need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
- 2× SuperEasyTM Mix-EDTA High blood tolerance: up to 45% in human blood and 20% in mouse blood.
The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.
Work Flow
Electropherogram
The blood direct PCR series kit can amplify the target gene from a variety of sources of anticoagulated whole blood as a template. 2× SuperEasyTM Mix-EDTA has strong tolerance to PCR reaction inhibitors, high detection sensitivity and strong amplification specificity. Use human blood as a template to amplify related genes and analyze by 1% agarose gel electrophoresis, as shown in the figure below:
Transpotation & Storage
The whole transportation process with low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
This kit should be stored at -20°C.
- 2× SuperEasyTM Mix-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
Contact Person: Maggie
Tel: +8615281067355
