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Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment

Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment

  • Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment
  • Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment
Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 50T, 200T, 500T, 2000T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment
Function: Extraction Type: Whole Blood Direct PCR
Application: EDTA Anticoagulated Whole Blood Operation Temperature: -20℃
High Light:

EDTA Blood Direct PCR Kits

,

ISO Extraction Direct PCR Kits

Strong amplification PCR system Blood SuperDirect PCR Kits With EDTA no pretreatment

 

Description

 

Blood SuperDirectTM PCR Kit-EDTA uses a unique PCR buffer system, which allows direct PCR with whole blood samples as a template without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.

2× SuperEasyTM Mix-EDTA contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.

 

Kit conponents

 

Blood SuperDirectTM PCR Kit-EDTA

EDTA Anticoagulant whole blood direct PCR kit

 

Kit composition

TP-0411T TP-04111 TP-04112 TP-04113
50 T 200 T 500 T 2000 T
2× SuperEasyTM Mix-EDTA 500μl 1ml×2 1.7ml×3 1.7ml×12
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Manual 1 1 1 1

 

Feature

 

- There is no need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.

- The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.

- 2× SuperEasyTM Mix-EDTA High blood tolerance: up to 45% in human blood and 20% in mouse blood.

The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.

 

Work Flow

 

 

Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment 0

 

Electropherogram

 

The blood direct PCR series kit can amplify the target gene from a variety of sources of anticoagulated whole blood as a template. 2× SuperEasyTM Mix-EDTA has strong tolerance to PCR reaction inhibitors, high detection sensitivity and strong amplification specificity. Use human blood as a template to amplify related genes and analyze by 1% agarose gel electrophoresis, as shown in the figure below:

 

Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment 1

 

Transpotation & Storage

 

The whole transportation process with low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

This kit should be stored at -20°C.

- 2× SuperEasyTM Mix-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.

Strong Amplification PCR System Blood SuperDirect PCR Kits With EDTA No Pretreatment 2

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
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Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
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