Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | TP-TP-0134T/01341/01342/01343 |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 50T, 200T, 500T, 2000T |
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| Type: | Mouse Tail Direct PCR | Application: | Extraction And Purification Of Genomic DNA From Fresh Or Frozen Large And Mouse Tails |
| Product Name: | Molecular Bio Animal Veterinary Diagnostic Laboratory Mouse Tail Direct PCR Kit With UNG | Key Word: | Direct PCR; DNA-Only Column |
| High Light: | Lab Mouse Tail Direct PCR Kit,Mouse Tail Direct PCR Kit UNG |
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Molecular bio Animal Veterinary Diagnostic Laboratory Mouse Tail Direct PCR Kit With UNG
Product Descprition
Mouse Tail Direct PCR Kit(65℃ lysis) -UNG uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.
2× M1-PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× M1-PCR EasyTM Mix (UNG), and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Kit contents:
| Mouse Tail Direct PCR Kit-UNG | |||||
| Kit contents | TP-0134T | TP-01341 | TP-01342 | TP-01343 | |
| 50T | 200T | 500T | 2000T | ||
| Part I | Buffer MP | 5ml | 20ml | 50ml | 100ml×2 |
| Foregene Protease Plus | 220μl | 880μl | 1.1ml×2 | 8.8ml | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Part II | 2× M1-PCR EasyTM Mix(UNG) | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| IFU | 1piece | 1piece | 1piece | 1piece | |
Features&advantages:
-No time-consuming and expensive DNA purification is required.
-The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.
-No special treatments such as grinding and crushing are required, and the operation is simple.
-The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
-Anti-pollution PCR system 2×M1-PCR EasyTM Mix (UNG), which effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.
Work Flow:
Storage and Shelf life:
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
Reagent 2×M1 PCR EasyTM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Contact Person: Maggie
Tel: +8615281067355
