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Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood

Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood

  • Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood
  • Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood
Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 50T, 200T, 500T, 2000T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Blood SuperDirect PCR Kit EDTA With UNG For Dentification Of Anticoagulated Whole Blood
Function: Direct PCR Identification Type: Blood Direct PCR
Application: EDTA Anticoagulated Whole Blood Operation Temperature: Room Temperature
High Light:

Super Direct PCR Kit EDTA

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Blood SuperDirect PCR Kit

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Direct PCR Kit EDTA UNG

Blood SuperDirect PCR Kit EDTA with UNG for dentification of anticoagulated whole blood

 

Description

 

Blood SuperDirectTM PCR Kit(EDTA) -UNG uses a unique PCR buffer system, which allows direct PCR with whole blood samples without DNA extraction or sample processing. EDTA, Heparin anticoagulated whole blood, and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can reach 45% of the PCR system, and that of mouse blood can reach 20%, which can sensitively amplify blood samples. Genomic and exogenous DNA fragments of interest.

2× SuperEasyTM Mix-EDTA contains our company's unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.

2× SuperEasyTM Mix (UNG) replaces dTTP with dUTP on the basis of 2× SuperEasyTM Mix, and at the same time adds a UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP. Before the PCR reaction, the PCR product containing uracil is degraded by UNG enzyme. UNG enzyme will not have any effect on the template that does not contain uracil, so as to ensure the specificity and accuracy of amplification and prevent the possibility of large-scale genetic testing. PCR product contamination problems arise.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions.

D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the different anticoagulants used, the products are divided into two categories: EDTA anticoagulation whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulation whole blood direct PCR kit series (BloodSuperDirectTM PCR Kit series). -Heparin); the corresponding kit can also be selected according to the experimental needs, see the selection of kit types for details.

 

Kit conponents

 

Blood SuperDirectTM PCR Kit(EDTA)-UNG

 

Kit composition

TP-0412T TP-04121 TP-04122 TP-04123
50 T 200 T 500 T 2000 T
2× SuperEasyTM Mix(UNG)-EDTA 500μl 1ml×2 1.7ml×3 1.7ml×12
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Manual 1 1 1 1

 

Feature

 

- No time-consuming and expensive DNA purification, no pretreatment, and whole blood as a PCR template.

- The system has strong amplification ability and can sensitively detect genomic and exogenous target DNA fragments in blood.

- 2× SuperEasyTM Mix(UNG)-EDTA High blood tolerance: up to 45% in human blood and 20% in mouse blood.

- The sample is completely closed, and there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix(UNG)-EDTA can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.

 

Application

 

Specifically designed for direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, rat blood, chicken blood, bird blood, cow blood, dog blood, etc.).

 

Kit Component information

 

- 2× SuperEasyTM Mix(UNG)-EDTA: On the basis of 2× SuperEasyTM Mix-EDTA, dUTP is used to replace dTTP, and at the same time, UNG enzyme that can degrade the template containing dUTP is added, which can effectively prevent the contamination of PCR amplification products. Contains D-Taq DNA Polymerase specially modified by Fuji Bio, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. For PCR reactions, simply add the appropriate anticoagulated whole blood, primers, and ddH2O to the corresponding 2× SuperEasyTM Mix(UNG)-EDTA for PCR reactions.

- 6× DNA Loading Buffer: This Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer provided with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large group of tail lights in the lane during electrophoresis, which will affect the experimental results.

 

Work Flow

Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood 0

 

Transportation

 

The whole transportation process with low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

 

Storage

 

This kit should be stored at -20°C.

- 2× SuperEasyTM Mix(UNG)-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.

Blood SuperDirect PCR Kit EDTA UNG For Dentification Of Anticoagulated Whole Blood 1

 

 

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
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