Place of Origin: | Sichuan, China |
Brand Name: | Foregene |
Certification: | CE, ISO |
Model Number: | TP-04111/04113 |
Minimum Order Quantity: | 1 kit |
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Price: | Negotiable |
Packaging Details: | craft paper bag |
Delivery Time: | 5-8 working days |
Payment Terms: | L/C, D/A, D/P, T/T |
Supply Ability: | 100000kits per month |
Factory: | Foregene | Product Name: | Blood SuperDirect PCR Kits With EDTA |
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Function: | Extraction | Type: | Blood Direct PCR |
Application: | EDTA Anticoagulated Whole Blood | Operation Temperature: | Room Temperature |
High Light: | Lab Reagent Blood Direct PCR Kits,Whole Blood Anticoagulated Direct PCR Kits,EDTA Blood Direct PCR Kit |
Lab reagent Blood Super Direct PCR Kits using whole blood anticoagulated with EDTA
Description
This product uses a unique PCR buffer system, without DNA extraction or sample processing, it can perform direct PCR with whole blood samples as a template. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system, and mouse blood can be up to 20%, which can sensitively amplify genomic and exogenous DNA fragments in blood samples.
2× SuperEasyTM Mix-EDTA contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants, the products are divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR) Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.
Kit conponents
Blood SuperDirectTM PCR Kit- EDTA | ||||
Content | TP-0411T | TP-04111 | TP-04112 | TP-04113 |
50T | 200T | 500T | 2000T | |
2× SuperEasyTM Mix-EDTA | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
IFU | 1 | 1 | 1 | 1 |
Feature
- No need for time-consuming and expensive DNA purification, no pre-treatment, and direct use whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
- 2×SuperEasyTM Mix-EDTA has high blood tolerance: up to 45% in human blood and 20% in mouse blood.
- The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results.
Application
Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).
Transportation
The whole process of low-temperature ice box transportation, to ensure the kit is in a state of <4 ℃.
Storage
This kit should be stored at -20°C.
- 2×SuperEasyTM Mix-EDTA, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer, can be stored at 4℃ or -20℃ for a long time.
Direct PCR example electropherogram
The blood direct PCR series kit can amplify the target gene from a variety of sources of anticoagulated whole blood as a template. 2×SuperEasyTM Mix-EDTA has strong tolerance to PCR reaction inhibitors, high detection sensitivity and strong amplification specificity. Use human blood as a template to amplify related genes and analyze by 1% agarose gel electrophoresis, see image below:
Contact Person: Maggie
Tel: +8615281067355