Plant Seed Direct PCR Kit I-UNG For plant seeds containing low polysaccharide and polyphenol components

Description:

Plant Seed Direct PCR Kit I-UNG uses a unique lysis reaction system, which can quickly release genomic DNA from plant seed samples with low polysaccharide and polyphenol content (such as: rice, wheat, tobacco, corn, soybeans, etc.) for use in PCR reactions, so it is particularly suitable Large-scale genetic testing. In order to meet the needs of different detection tests, this kit can use multiple types of samples (such as whole seeds, small tissue cuts, or ground samples of multiple seeds) for experiments. Among them, for seed samples that still need to be germinated, you can choose to cut out micro-tissue cuts (1-5mg) other than the seed embryo for experiment; for experiments that need to be sampled in a large number of samples, you can choose to mix multiple samples and combine them. After grinding into particles with a diameter of about 0.5mm, the experiment can be carried out (at least 0.1% of the target sample can be detected).

The process of releasing genomic DNA from the cleavage reaction system can be completed within 5-10 minutes. There is no need for other processes to remove protein, RNA or secondary metabolites, and the released trace amounts of DNA can be used as a template for PCR reactions.

2× Seed PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can directly use the lysis mixture of plant materials as a template for efficient and specific amplification. This reagent contains the company's D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, and stabilizer specially modified for direct PCR. Used in conjunction with the lysis reaction system can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.

2× Seed PCR Easy^TM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× Seed PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

According to the differences in plant samples, the products are divided into two categories: Plant Seed Direct PCR Kit and Plant Seed Direct PCR Plus Kit. In the lysis stage, considering that different types of seeds or tissue materials vary greatly in the amount of reagents used in the lysis process, customers can choose the corresponding plant seed (small and medium) direct PCR kit or plant seed (large) according to the size of their experimental materials Direct PCR kit; in the PCR detection stage, customers can also choose ordinary 2× Seed PCR EasyTM Mix or 2× Seed PCR Easy^TM Mix (UNG) which can prevent the contamination of PCR amplification products according to the experimental needs.

Specifications:

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

Kit components:

Features&advantages:

- No need time-consuming and expensive DNA purification is required.

- The sample demand is small, just take a single seed.

- No special treatments such as grinding and crushing are required, and the operation is simple.

- The optimized PCR system enables PCR to have higher specificity and stronger tolerance to PCR reaction inhibitors.

- Anti-pollution PCR system 2× Seed PCR EasyTM Mix (UNG) can effectively eliminate the pollution caused by PCR products and ensure the specificity and accuracy of amplification.

Kit parameters:

Application: Identification of transgenic plants, genotyping, etc.

Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.

Sample size: Single whole seed, 1-100mg plant tissue

Detection range: PCR product ≤1kb

Application

- Scope of application: a variety of plant seeds.

- DNA released from sample lysis: only used as a PCR template.

- The kit can be used for the following purposes: identification of transgenic seeds, plant genotyping, etc.

Kit composition information

          - Buffer SP1:Provides the environment required for the lysis reaction of plant seeds.

- Buffer SP2:Neutralize the lysate so that it does not affect the subsequent PCR reaction.

-2× Seed PCR Easy^TM Mix(UNG):Contains special modified Taq DNA Polymerase, UNG enzyme, dNTPs, dUTP, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. During the PCR reaction, just add the appropriate lysis mixture, primers, and ddH2O to the 2× Seed PCR EasyTM Mix (UNG) to be used for the PCR reaction.

-6× DNA Loading Buffer:The Loading Buffer does not contain SDS. It is recommended to use the 6× DNA Loading Buffer included with the kit when performing agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use Loading Buffer containing SDS, otherwise there will be a large tail of bright light in the lane during electrophoresis, which will affect the experimental results.

Work Flow:

Electropherogram:

Storage and Shelf life:

-Part I of this kit should be stored at room temperature or 2-8℃.

vReagents Buffer SP1, Buffer SP2, 6× DNA Loading Buffer, under dry conditions, can be stored for 12 months. If you need to store the kit for a longer time, you can store it at 2-8℃.

-Part II of this kit should be stored at -20℃.

vReagent 2× Seed PCR Easy Mix, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

APPENDIX I

The comparison between Direct PCR and traditional PCR: