Send Message
Tel:
Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

CHINA FOREGENE,WORLD'S FOREGENE!

Home ProductsDirect PCR Kits

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants

  • Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants
  • Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants
  • Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants
  • Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants
Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO CE
Model Number: Cat.No.TP-0311T/03111/03112/03113(I) Cat.No.TP-0313T/03131/03132/03133(II)
Payment & Shipping Terms:
Minimum Order Quantity: 100 kits
Price: Negotiable
Packaging Details: Packing with safe trans carton
Delivery Time: 5-8 days
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100000 kits per month
Contact Now
Detailed Product Description
Product Name: Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants Type: Lab Test Kits
Package: Box Feature: Fast And Simple
Application: Pland Seed PCR Test Oem: Accept
Factory: FOREGENE Shelf Life: 1 Year
Storage Conditions: Part I:4-8℃; Part II: -20℃
High Light:

Transgenic Plant Seed Direct PCR Kit

,

Iso Pcr Kit Lysis Buffer

Plant Seed Direct PCR Kit I/II pcr kit lysis buffer ce iso loading buffer for transgenic plants

 

 

Description:

 

Plant Seed Direct PCR Kit adopts a unique cleavage reaction system, which can rapidly release genomic DNA from plant seed samples with low polysaccharide and polyphenol content (such as: rice, wheat, tobacco, corn, soybean, etc.) for PCR reaction, so it is especially suitable for Large-scale genetic testing. In order to meet the needs of different detection experiments, this kit can use various types of samples (such as whole seeds, micro-tissue cut or ground samples of multiple seeds) for experiments. Among them, for seed samples that still need to be germinated, micro-tissue pieces (1-5 mg) other than seed embryos can be selected for experiments; for experiments that require sampling and testing in a large number of samples, multiple samples can be mixed and combined. After grinding into particles with a diameter of about 0.5mm, the experiment is carried out (at least 0.1% of the target sample can be detected).

The cleavage reaction system can release genomic DNA within 5-10 minutes, without the need for other processes of deproteinization, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction.

2× Seed PCR EasyTM Mix has a strong resistance to PCR reaction inhibitors, and can directly use the lysis mixture of plant material as a template for efficient and specific amplification. This reagent contains the company's D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer specially designed for direct PCR modification. It can be used in combination with the cleavage reaction system to detect samples quickly and easily, and has the characteristics of high sensitivity, strong specificity and good stability.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems. The amplification speed can reach 2Kb/min. It is especially suitable for Direct PCR reaction.

According to the differences of plant samples, the products are divided into two categories: Plant Seed Direct PCR Kit and Plant Seed Direct PCR Plus Kit. In the lysis stage, considering that different types of seeds or tissue materials have large differences in the amount of reagents used in the lysis process, customers can choose the corresponding plant seeds (small and medium-sized) direct PCR kit or plant seeds (large) according to the size of their experimental materials. Direct PCR kit; in the PCR detection stage, customers can also choose the common 2× Seed PCR EasyTM Mix or the 2× Seed PCR EasyTM Mix (UNG) which has the effect of preventing the contamination of PCR amplification products according to the experimental needs.

 

 

Specifications:

 

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

 

 

Kit components:

 

Plant Seed Direct PCR Kit I

 

 

Kit component

TP-0311T TP-0311 1 TP-03112 TP-03113
50 T 200 T 500 T 2000 T

Part I

Buffer SP1 10ml 40ml 100ml 400ml
Buffer SP2 3ml 10ml 25ml 100ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Part II 2× Seed PCR EasyTM Mix 500μl 1ml×2 1.7ml×3 1.7ml×12
Instructions 1 piece 1 piece 1 piece 1 piece

 

Plant Seed Direct PCR Kit II

 

Kit components

TP-0313T TP-03131 TP-03132 TP-03133
50 T 200 T 500 T 2000 T

Part I

Buffer SP1 30ml 120ml 100ml×3 400ml×3
Buffer SP2 3ml 10ml 25ml 100ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Part II 2× Seed PCR EasyTM Mix 500μl 1ml×2 1.7ml×3 1.7ml×12
Instructions 1 piece 1 piece 1 piece 1 piece

 

Features&advantages:

 

-No time-consuming and expensive DNA purification(Time, labor and cost saved!)

-Less material

-Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding

-2 X Mix—reducing sample loading errors and reaction system preparation time

-Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes

-High throughput—lysis reaction can be completed in 96-well PCR plate

 

 

Kit parameters:

 

Application: Identification of transgenic plants, genotyping, etc.

Sample: Plant seeds or tissues with low polysaccharide and polyphenol content.

Sample size: Single whole seed, 1-100mg plant tissue

Detection range: PCR product ≤1kb

 

 

Work Flow:

 

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants 0

 

Electropherogram:

 

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants 1

 

Storage and Shelf life:

-Part I of this kit should be stored at room temperature or 2-8℃.

vReagents Buffer SP1, Buffer SP2, 6× DNA Loading Buffer, under dry conditions, can be stored for 12 months. If you need to store the kit for a longer time, you can store it at 2-8℃.

-Part II of this kit should be stored at -20℃.

vReagent 2× Seed PCR Easy Mix, if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

 

APPENDIX I

The comparison between Direct PCR and traditional PCR:

 

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants 2

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants 3

 

Plant Seed Direct PCR Kit I/II Pcr Kit Lysis Buffer Ce Iso Loading Buffer For Transgenic Plants 4

 

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
Other Products
Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
Mobile Site Privacy Policy | China Good Quality PCR Kits Supplier. © 2022 - 2024 foregenebio.com. All Rights Reserved.