Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 200T, 500T, 2000T |
| Minimum Order Quantity: | 100kits |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Function: | Extraction |
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| Type: | Blood Direct PCR | Application: | Direct PCR Identification Of Heparin Anticoagulated Whole Blood. (Including: Human Blood, Mouse Blood, Chicken Blood, Bird Blood, Cow Blood, Dog Blood, Etc.). |
| Operation Temperature: | Room Temperature | Product Name: | Blood SuperDirectTM PCR Kit(UNG)-Heparin DNA Polymerase PCR Kit PCR Products |
Blood SuperDirect PCR Kits(UNG) use whole blood anticoagulated with EDTA
Description
This product uses a unique PCR buffer system, without DNA extraction or sample processing, it can perform direct PCR with a whole blood sample as a template. EDTA, Heparin anticoagulated whole blood and blood collection cards (such as Whatman 903® and FTA® blood collection cards) can be directly used for PCR amplification and identification, which greatly shortens the detection time. The 2× SuperEasyTM Mix provided by this kit has strong inhibitor tolerance. The maximum amount of human blood used as a PCR template can be up to 45% of the PCR system and 20% of mouse blood, which can sensitively amplify blood samples. Genomic and foreign target DNA fragments.
2× SuperEasyTM Mix-EDTA contains our company’s unique Foregene D-Taq DNA Polymerase, dNTPs, MgCl2, Taq Reaction Buffer, PCR reaction enhancer, optimizer and stabilizer.
2× SuperEasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× SuperEasyTM Mix, and at the same time adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
According to the different anticoagulants used, this product is divided into two categories: EDTA anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-EDTA) and Heparin anticoagulant whole blood direct PCR kit series (Blood SuperDirectTM PCR Kit-Heparin); You can also choose the corresponding kit according to the needs of the experiment, see the selection of kit type for details.
Kit conponents
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Blood SuperDirectTM PCR Kit(UNG)-EDTA EDTA Anticoagulated Whole Blood Direct PCR Kit-UNG |
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| Kit composition | TP-0412T | TP-04121 | TP-04122 | TP-04123 |
| 50 T | 200 T | 500 T | 2000 T | |
| 2× SuperEasyTM Mix(UNG)-EDTA | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 |
| Manual | 1 | 1 | 1 | 1 |
Feature
- There is no need for time-consuming and expensive DNA purification, no pretreatment, and direct use of whole blood as a PCR template.
- The system has strong amplification ability and can sensitively detect the genome and foreign target DNA fragments in the blood.
- 2× SuperEasyTM Mix(UNG)-EDTA has high blood tolerance: up to 45% in human blood and 20% in mouse blood.
- The sample is fully enclosed, so there is no need to worry about sample contamination and false positive PCR results. Anti-pollution PCR system 2×SuperEasyTM Mix(UNG)-EDTA, effectively eliminates the pollution caused by PCR products, and ensures the specificity and accuracy of amplification.
Application
Dedicated to direct PCR identification of EDTA anticoagulated whole blood. (Including: human blood, mouse blood, chicken blood, bird blood, cow blood, dog blood, etc.).
Transportation
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
Storage
This kit is stored at -20°C.
- 2× SuperEasyTM Mix(UNG)-EDTA,If used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).
- 6× DNA Loading Buffer,It can be stored at 4°C or -20°C for a long time.
Work Flow
Contact Person: Maggie
Tel: +8615281067355
