Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | TP-01331/01313 |
| Minimum Order Quantity: | 1 kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 200 ×20μl Rxns,2000×20μl Rxns |
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| Type: | Mouse Tail Direct PCR | Application: | Extraction And Purification Of Genomic DNA From Fresh Or Frozen Large And Mouse Tails |
| Product Name: | High DNA Extraction Yield Mouse Tail SuperDirect PCR Kits For Mouse Direct PCR Perform Room Temperature Lysis | Key Word: | Direct PCR; High DNA Extraction Yield |
High DNA extraction yield Mouse Tail SuperDirect PCR Kits for mouse direct PCR perform room temperature lysis
Product Descprition
This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.
2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Product INFO:
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time |
60-90min (24 samples) |
| Centrifuge | Table centrifuge | Tissue enzymolysis isolation | centrifugal isolation |
| Purification column DNA load carrying capacity | 80μg | Liquid volume of spin column | 800μl |
| Elution volume | 100-200μl |
Mouse tail sample processing volume |
1-5ml |
Kit contents:
| Mouse Tail SuperDirectTM PCR Kit | |||||
| Kit contents | TP-0131T | TP-01311 | TP-01312 | TP-01313 | |
| 50T | 200T | 500T | 2000T | ||
| Part I | Buffer NP | 3ml | 10ml | 25ml | 100ml |
| Foregene Protease Plus I | 55μl | 220μl | 550μl | 1.1ml×2 | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Part II | 2× M-PCR EasyTM Mix | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| IFU | 1piece | 1piece | 1piece | 1piece | |
Features&advantages:
-No time-consuming and expensive DNA purification is required.
-The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.
-Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.
-Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.
-Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Genomic DNA fragment size:
Mouse Tail DNA Mini Kit uses a silica membrane column to isolate and purify mouse tail genomic DNA. The purified genomic DNA fragments size are all around 23 kb. As shown in the figure:
Storage and Shelf life:
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease Plus I has a unique formula. To ensure its better activity and stability, please store it at 4℃.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
Reagent 2×M-PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Work Flow:
Contact Person: Maggie
Tel: +8615281067355
