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Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits

Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits

  • Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits
  • Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits
  • Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits
Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 5T, 25T, 50T, 100T, 250T
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Fast Lab Use Soil DNA Isolation Kits
CAT NO.: DE-05511, DE-05512, DE-05513, DE-05514, DE-05515 Type: DNA-Only Column
Application: Purification Of Genomic DNA Operation Temperature: Room Temperature
High Light:

Quickly Purify DNA Isolation Kits

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100T Soil DNA Isolation Kit

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Soil DNA Isolation Kit 50 T

Quickly purify and obtain high-quality genomic DNA from various soil sample microorganisms Soil DNA Isolation Kits

 

 

Description

 

This kit provides a quick and easy method for extracting genomic DNA from various sources of soil. There are a large number of inhibitors such as humic acid and metal ions in soil samples. Even if these substances are present in small amounts in the purified DNA, they will have an impact on downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purifying soil DNA is how to effectively remove inhibitors in the soil.

This kit uses DNA-Only Column that can specifically bind to DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors from the soil without organic solvent extraction or ethanol and isopropanol precipitation. The extraction of DNA from soil samples can be completed in 90 minutes.

 

Obtain Genomic DNA From Various Soil Sample Microorganisms Soil DNA Isolation Kits 0

 

Kit conponents

 

Soil DNA Isolation Kit
Kit contents DE-05511 DE-05512 DE-05513 DE-05514 DE-05515
5 T 25 T 50 T 100T 250T
Buffer SG1 3.5ml 18ml 35ml 70ml 180ml
Buffer SG2 300μl 1.5ml 3ml 6ml 15ml
Buffer SG3 3.5ml 18ml 35ml 70ml 180ml
Buffer SG4 120μl 600μl 1.2ml 2.4ml 6ml
Buffer PW 3ml 15ml 30ml 60ml 150ml
Buffer WB 2.5ml 12.5ml 25ml 50ml 125ml
Buffer EB 1.5ml 6ml 15ml 60ml 120ml
Buffer TE 6ml 30ml 60ml 120ml 300ml
Foregene Protease 280μl 1.4ml 1.4mlx2 5.6ml 14ml
DNA-Only Column 5 sets 25 sets 50 sets 100 sets 250 sets
IFU 1 piece 1 piece 1piece 1 piece 1 piece
 

 

Features&advantages

 

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: The operation is simple, and the soil genomic DNA extraction operation can be completed within 90 minutes.

- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: No organic reagent extraction is required.

- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Genomic DNA Application

 

The genomic DNA purified by Soil DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.

 

Quality Control

 

According to FOREGEN’s Total Quality Management System, each batch of Soil DNA Isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

DNA-Only Column Characters

 

Maximum binding capacity 80μg
Maximum loading volume 800μl
Longset DNA fragment 23kb
Minimum elution volume* 100μl
Selection of samples Newly taken soil samples
Maximum amount of starting material* 5ml soil sample

*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery rate and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate; if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.

 

 

Genomic DNA extraction yield and purity

 

The amount of soil genomic DNA extracted is related to factors such as the source, storage time, and moisture content of the soil sample. Process soil samples from various sources according to the Soil DNA Isolation Kit, and the amount and purity of genomic DNA obtained are shown in the following table:

Sample source Sample dosage(mg) DNA yield(μg) OD260/280 OD260/230
Flower bed soil 500 4-5 1.7-1.9 1.7-1.9
Grassland soil (stony) 500 4-6

1.7-1.9

1.8-1.9
Forest soil 500 4-6 1.7-1.9 1.7-1.8

Lawn soil

500 6-10 1.7-1.8 1.8-2.0
Lakeside 500 6-10

≈1.8

1.9-2.0
Pine forest 500 8-12 ≈1.8 1.8-1.9
Pond silt 500 6-8 1.7-1.8 1.7-1.8

Note: The data in this table is for reference only. In actual operation, due to the storage conditions of the materials used, operating proficiency and other factors, the data obtained may differ slightly from the data in this table.

 

Storage and Shelf life

 

- This kit can be stored for 12 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

- Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

- Dry Lysozyme powder is stored at -20°C; the prepared Lysozyme solution is divided into small portions and stored at -20°C.

Contact Details
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Contact Person: Maggie

Tel: +8615281067355

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