Lab Reagent Mouse Tail DNA Mini Kit For Genomic DNA Purification In 1 Hour

High quality lab reagent Mouse Tail DNA Mini Kit for genomic DNA purification in 1 hour​

Product Descprition

Due to the structure and properties of the mouse tail itself, the extraction of mouse tail genomic DNA has always troubled researchers: one is that the processing time of the mouse tail is long and the genomic DNA is easily degraded, the other is difficult to handle and the purity of the extracted genomic DNA is low.

High DNA extraction yield and purity Mouse Tail Direct PCR Kit without prior DNA purification uses a DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can digest mouse tail samples within 45 minutes, thereby minimizing the degradation of genomic DNA and shortening sample processing time. The kit can extract 10-20μg of high-quality, high-purity genomic DNA from 0.5-1cm juvenile or adult mouse tails within 60-90min.

The DNA-only silica gel matrix material used in the spin column is a unique new material of the company, which can efficiently and specifically adsorb DNA. The maximum adsorption capacity for DNA is 80μg. The unique buffer and elution system can maximize the removal of RNA, Impurity proteins, ions and other organic compounds in the cell. The extracted genomic DNA fragment is large, high in purity, stable and reliable in quality, and the size of the DNA fragment is stable at about 23kb.

The kit can also be used to extract other mouse tissues outside the mouse tail, including mouse ears, mouse muscle, mouse liver and other tissues, so that one kit can be used for multiple purposes and meet the needs of users for different experiments.

Kit Content:

*: Buffer TL2 and Buffer PW contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.

Features

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.

- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: No organic reagent extraction is required.

- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Kit Application

It is suitable for the extraction and purification of genomic DNA from fresh or frozen large and mouse tails (also used to purify mouse or rat muscle and mouse ear genomic DNA).

DNA-Only Column Characters

*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery efficiency and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate; if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.

Work Flow: