Lab Reagent General Plasmid Mini DNA Isolation Kit No RNase Contamination

Safe and fast plasmid DNA purification lab reagent General Plasmid Mini DNA Isolation Kit no RNase contamination
 
 
Description:
 
The FOREGENE’S nucleic acid extraction and purification series products can be extracted and purified to high-quality nucleic acids from a variety of sources and complex component samples. The DNA extraction series products are based on DNA-Only silica membrane purification columns and unique formula reagents that can purify high-quality DNA from a variety of samples.
The General Plasmid Mini Kit uses DNA-Only purification column technology and an efficient SDS lysis formula to obtain high-quality plasmid DNA from bacteria within 20 minutes. The DNA-Only silica gel membrane used in the spin column is a unique new material of FOREGENE, which can adsorb DNA efficiently and specifically,and remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum binding capacity of plasmid DNA of DNA-Only purification column is 60μg DNA. The amount of plasmid DNA actually extracted is related to the volume of the bacterial solution, the number of plasmid copies (high copy, low copy), strains, culture conditions and other factors.

 
Kit components:
 

 
Features&advantages:
 
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA in the plasmid DNA without adding RNase during the experiment, avoiding the contamination of the laboratory by exogenous RNase.
-Fast speed: Plasmid DNA extraction can be completed within 20 minutes.
-Safe: No organic reagent extraction is required.
-High quality: The plasmid obtained by extraction has no RNA, no RNase and extremely low ion content, which can meet the requirements of various experiments.
 
Plasmid DNA parameters:
 
The plasmid DNA purified by the General plasmid mini kit has high purity and can be used for routine molecular biology operations, such as: transformation, restriction enzyme digestion, PCR, library construction, transfection (transfection of subculture cells), sequencing (it is recommended to use ddH₂O to elute plasmids) DNA).
 
Plasmid DNA extraction yield rate:
 
The amount of the extracted plasmid DNA is related to the bacterial culture concentration, copy number, strain, culture conditions and other factors; the obtained plasmid DNA is of high purity,OD260/280=1.7-1.9.

Note: The above data are for reference only, the actual yield is related to bacterial culture conditions, culture time, operation, etc.
 
DNA-Only Column Characters:
 

 
 
Storage and Shelf life:
 
This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.