Lab Reagent Mouse Tail Direct PCR Kit By DNA Only Column Without RNase Contamination

Fast speed lab reagent Mouse Tail Direct PCR Kit by DNA-Only Column without RNase contamination

Product Descprition

Fast speed lab reagent Mouse Tail Direct PCR Kit by DNA-Only Column without RNase contamination uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× M1-PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Kit contents:

*: Buffer TL2 and Buffer PW contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.

Features&advantages:

• No time-consuming and expensive DNA purification is required.
• The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.
• No special treatments such as grinding and crushing are required, and the operation is simple.
• The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit Application:

It is suitable for the extraction and purification of genomic DNA from fresh or frozen large and mouse tails (also used to purify mouse or rat muscle and mouse ear genomic DNA).

Genomic DNA Application:

The genomic DNA purified by the Mouse Tail DNA Mini Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern blotting, library construction and other experiments.

Kit parameters:

Application: Mouse tail, mouse ears, muscle and other tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

DNA-Only Column Characters:

*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery efficiency and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate; if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.

Storage and Shelf life:

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

 Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

 Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

 Reagent 2×M1 PCR Easy^TM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).