Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1kit |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | High Quality DNA Isolation Kits For Genomic DNA Purification From Whole Blood |
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| CAT NO.: | DE-05111, DE-05112, DE-05113 | Type: | DNA-Only Column |
| Application: | Whole Blood Genomic DNA | Operation Temperature: | Room Temperature |
| High Light: | Genomic DNA Purification DNA Isolation Kits,ISO DNA Isolation Kits |
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Description
This kit adopts new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulant blood samples in a short period of time, thus avoiding DNA degradation to the maximum degree and obtaining the maximum amount of genomic DNA.The rapid blood processing system and the simple centrifugal column operation greatly simplify the extraction of blood genome, making the genomic DNA of high quality and high purity can be obtained within 40 minutes.
The DNA-only silica gel membrane used in the centrifuge column of this kit is a new type of material unique to our company, which can adsorb DNA efficiently and specifically, and can remove RNA, impurities, proteins, ions and other organic compounds in cells to the maximum extent.The obtained DNA fragments are large, high purity, stable and reliable quality. The maximum carrying capacity of a centrifugal column is 80μg DNA.The obtained DNA can be used in molecular biology experiments such as enzyme digestion, PCR, Southern hybridization and library construction.
The kit can process up to 1ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.
Kit conponents
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Buffer BL1 |
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Buffer BL2 |
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Buffer DC |
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Buffer PW |
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Buffer WB1 |
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Buffer EB |
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Foregene Protease Plus |
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DNA-Only Column |
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Insctiontrus |
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.
- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.
- Convenience: The centrifugation is performed at room temperature, 4°C centrifugation or ethanol precipitation of DNA is not required.
- Safety: No organic reagent extraction is used.
- High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit application
It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.
Workflow
Precautions
- EDTA is recommended as a blood anticoagulant.
- Repeated freeze-thaw should be avoided, otherwise the extracted DNA fragments will be smaller and the extracted amount will decrease.
- Before use, carefully check whether there is precipitation in Buffer BL1, Buffer BL2 and Buffer PW. If there is precipitation, please dissolve it at 37℃ and mix well before use.
- Before using the kit, be sure to check whether anhydrous ethanol has been added to Buffer WB1 as instructed.60 mL anhydrous ethanol (DE-05111) was added to Buffer WB1 before use.
- Elution volume: Buffer EB should not be less than 100 μ L, otherwise it will affect DNA production.
- Remember not to add RNA enzymes to any Buffer.
- All centrifuges are centrifuged at room temperature (15-25℃).
- All experimental steps were carried out at room temperature (15-25℃).
Samples
- Non-nuclear anticoagulant blood: single treatment, dosage should not exceed 1 ml.
- Nuclear anticoagulant blood: single treatment, dosage do not exceed 50 μl.
Storage and Shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
The Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months); it will be more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.
Contact Person: Maggie
Tel: +8615281067355
