Place of Origin: | China |
Brand Name: | Foregene |
Model Number: | TP-01341/ TP-01343 |
Minimum Order Quantity: | 100kits |
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Price: | Negotiable |
Packaging Details: | craft paper bag |
Delivery Time: | 5-8 working days |
Payment Terms: | L/C, D/A, D/P, T/T |
Supply Ability: | 100000kits per month |
Factory: | Foregene | Specifications: | 200 ×20μl Rxns,2000×20μl Rxns |
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Type: | Mouse Tail Direct PCR-UNG | Application: | MOUSE TAIL |
Product Name: | Quick And Easy Mouse Tail Direct PCR Kit-UNG One Step No Need DNA Extraction | Key Words: | Direct PCR; Mouse Tail |
High Light: | Foregene Direct PCR Kit UNG,Direct PCR Kit UNG 200x 20ul rxns,Direct PCR Kit one step 2000x 20ul rxns |
Quick and easy Mouse Tail Direct PCR Kit-UNG one step no need DNA extraction
Product Descprition
This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.
2× M1-PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× M1-PCR EasyTM Mix (UNG), and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Product components:
Part I | Buffer MP |
Foregene Protease Plus | |
6× DNA Loading Buffer | |
Part II | 2× M1 PCR EasyTM Mix(UNG) |
Instructions |
Features&advantages:
Kit parameters:
Scope of application: mouse tail, mouse ear, muscle and other tissues.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.
Product quality control:
In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Mouse Tail Direct PCR kit-UNG is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Storage and Shelf life:
Part I of this kit should be stored at 2-8℃.
The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
Reagent 2×M1 PCR EasyTM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Contact Person: Foregene
Tel: +8613880676215