Foregene Protease Direct PCR Kits Master Mix 200*20ul Rxns 2000*20ul Rxns

Fast and efficient Animal Tissue Direct PCR kits without DNA Isolation

Product Descprition

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× PCR Easy^TM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Product components:

Features&advantages:

• No time-consuming and expensive DNA purification
• The sample demand is small, as little as 5mg animal tissue can be tested.
• No special treatment such as grinding and crushing is required, and the operation is simple.
• Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Kit parameters:

Application: a variety of animal tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Animal Tissue Direct PCR kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Storage and Shelf life:

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×PCR Easy^TM Mix can be stored for 12 months at -20℃ ; if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).