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Room Temperature Operation Viral DNA RNA Isolation Kit High Yield

Room Temperature Operation Viral DNA RNA Isolation Kit High Yield

  • Room Temperature Operation Viral DNA RNA Isolation Kit High Yield
  • Room Temperature Operation Viral DNA RNA Isolation Kit High Yield
  • Room Temperature Operation Viral DNA RNA Isolation Kit High Yield
  • Room Temperature Operation Viral DNA RNA Isolation Kit High Yield
Room Temperature Operation Viral DNA RNA Isolation Kit High Yield
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO CE
Model Number: DR-01012
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: Packing with safe trans carton
Delivery Time: 5-8 days
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100000 kits per month
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Detailed Product Description
Product Name: High Purification And RNA Yield DNA RNA Isolation Kit With RNase Free Type: Spin Column Technology
Packing: 100 Tests Per Kit OEM: Accept
Feature: Large Sample Processing Capacity—up To 200μl Samples Can Be Processed Each Time. MOQ: 1 Kit
High Light:

OEM Viral DNA RNA Isolation Kit

,

CE Spin Column RNA Isolation Kit

Room temperature operation Viral DNA RNA Isolation kit high yield

 

 

Description:

 

The Viral DNA and RNA Isolation Kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality viral DNA and RNA from samples such as plasma, serum, cell-free body fluid, and cell culture supernatant. The kit specifically adds Linear Acrylamide, which can easily capture small amounts of DNA and RNA from the samples. DNA/RNA-Only Column can efficiently bind DNA and RNA. The kit can process a large number of samples at the same time.

 

The entire kit does not contain RNase, so the purified RNA will not be degraded. Buffer RW1 and Buffer RW2 can ensure that the obtained viral nucleic acid free of protein, nuclease or other impurities, which can be used directly for downstream molecular biology experiments.

 

Specifications:

 

50 Preps, 100 Preps, 200 Preps

 

Kit component

 

Viral DNA/RNA Isolation Kit
Kit component DR-01011 DR-01012 DR-01013
50 T 100 T 200 T
Linear Acrylamide 120μl 240μl 480μl
Buffer DRL* 25ml 50ml 100ml
Buffer RW1* 25ml 50ml 100ml
Buffer RW2 24ml 48ml 96ml
RNase-Free ddH2O 10ml 20ml 40ml
DNA/RNA Column 50 100 200
Instructions 1 1 1

* :Buffer DRL and Buffer RW1 contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.

 

Product information

 

Type Centrifugal column Purification preparation Foregene centrifugal column,reagent
Flux 1-24 samples Preparation time ~20min (24 samples)
Centrifuge Desk centrifuge Maximum liquid capacity of centrifugal column 800μl

Load capacity of RNA

Purification column

20μg

Size of single sample

Handling

≤200μl
Minimum volume of elution 30-50μl
 

 

Features&advantages:

 

-No need to worry about RNA degradation. The whole kit is RNase-Free

-Simple—all operations are completed at room temperature

-Fast—operation can be completed in 20 minutes

-Safe—no organic reagent used

-Large sample processing capacity—up to 200μl samples can be processed each time.

-High quality—the purified DNA and RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

 

Kit application:

 

Viral DNA and RNA Isolation Kit is suitable for the extraction and purification of viral nucleic acid in samples such as plasma, serum, cell-free body fluid and cell culture supernatant.

 

 

Storage and Stability

 

uThe kit can be stored for 12 months under dry conditions at room temperature (15–25°C); it can be stored at 2–8°C if it needs to be stored for a longer time.

Note: If stored at low temperature, the solution is prone to precipitation. Be sure to place the solution in the kit at room temperature for a period of time before use. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix well before use.

u Linear Acrylamide solution can be stored for 7 days at room temperature; please take out the kit and store it at -20after receipt.

u After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8°C for up to 48 hours. Please use it for immediate use

 

Product Quality Control

 

In accordance with FOREGENE's Total Quality Management System, each batch of viral DNA/RNA extraction kits is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

Kit Component Information

u Linear Acrylamide: Reduce the background binding of the purification column and capture the trace nucleic acids in the system.

u Buffer DRL: Provides the environment required for sample lysis and the environment for passing the column.

u Buffer RW1: Remove impurities such as proteins in nucleic acids.

u Buffer RW2: Remove residual salt ions from nucleic acids.

u RNase-Free ddH2O: Elute nucleic acids on the purification column membrane.

u DNA/RNA Column: Highly efficient adsorption of nucleic acids.

 

 

DNA/RNA Column features

Maximum Nucleic acid binding capacity

Room Temperature Operation Viral DNA RNA Isolation Kit High Yield 0

20μg
Maximum loading volume 800μl

Minimum elution volume1*

Room Temperature Operation Viral DNA RNA Isolation Kit High Yield 1

30μl
Selection of samples Plasma, serum, cell-free body fluid or cell culture supernatant, etc.
Maximum amount of starting material 2* 200μl

 

1*: The minimum elution volume of 30μl is a reasonable recommended volume given in consideration of nucleic acid recovery rate and concentration. If to increase the yield of nucleic acid, the volume of the eluate can be appropriately increased; if in order to increase the concentration of the purified nucleic acid, a part of the nucleic acid yield is sacrificed,under the premise that the elution volume is appropriately reduced, such as using an elution volume of 15μl, a higher concentration of nucleic acid can be obtained.

2*: For larger sample size, please use multiple DNA/RNA Columns for nucleic acid purification.

 

Nucleic acid extraction yield and purity

 

Viral DNA/RNA Isolation Kit can be used to purify viral nucleic acids from samples such as plasma, serum, cell-free body fluids, and cell culture supernatants. The yield is related to the sample itself, initial sample volume, sample freshness, sample storage time and operation related. The purified nucleic acid has OD260/280=1.7-2.1.

 

 

Work Flow:

Room Temperature Operation Viral DNA RNA Isolation Kit High Yield 2

Diagram:

Room Temperature Operation Viral DNA RNA Isolation Kit High Yield 3

Precautions:

 

- Repeated freezing and thawing of the sample should be avoided, otherwise the extracted viral nucleic acid will be degraded and the extraction amount will also decrease.

- The sample processing volume should not exceed 200μl, otherwise the yield and purity of viral nucleic acid will be affected.

- After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8°C for up to 48h. Please use it now.

- Please add absolute ethanol to Buffer RW2 before using the kit. Please refer to the label on the reagent bottle for the added amount.

The addition amount of anhydrous ethanol in the kits of different specifications is shown in the following table:

Product specifications Amount of anhydrous ethanol added
DR-01011 60ml
DR-01012 120ml
DR-01013 240ml

- The nucleic acid yield and quality are related to the elution volume and sample processing volume. It is recommended to use 200 μl of sample volume per 500 μl of Buffer DRL.

- Elution volume: The elution volume should not be less than 30μl, otherwise it will affect the nucleic acid recovery efficiency.

- All experimental steps are carried out at room temperature (15-25℃) unless otherwise specified.

- Please check whether there is crystal precipitation in Buffer DRL and Buffer RW1 in the kit. If there is crystal precipitation after storage at low temperature, you can place the Buffer at room temperature or 37°C for a period of time, dissolve the crystal and mix well before use.

 

Storage and Shelf life:

- The Viral DNA and RNA Isolation Kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time.

-Linear Acrylamide solution can be stored at room temperature for 7 days. After receiving the kit, please take Linear Acrylamide solution out and store it at -20℃.

-After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8℃ for up to 48h. Please use the fresh prepared solution.

 

 

 

Safety

- This product is for scientific research use only, please do not use it for medicine, clinical medicine, food and cosmetics.

- When using the kit, please wear lab coats, disposable latex gloves, disposable masks, etc. to protect yourself; and avoid artificially introduced RNase contamination to the greatest extent possible.

-  Buffer DRL contains chaotropic salts: denaturant, irritant.

- Buffer RW1 contains chaotropic salts: denaturant, irritant.

- Buffer RW2 contains ethanol: Flammable.

 

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
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