Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 5T, 50T, 100T, 200T |
| Minimum Order Quantity: | 1 BOX |
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| Price: | Negotiate |
| Delivery Time: | 1-7 days |
| Payment Terms: | T/T, L/C, Western Union |
| Supply Ability: | 10000 BOX per Month |
| Product Name: | Spin Column Animal MiRNA Isolation Kits RE-0101T For Laboratory Research Use | CAS No.: | RE-0101T, RE-01011, RE-01012, RE-01014 |
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| Application: | Animal Tissues Or Cells | Classification: | General Reagents |
| Purity: | 99.9% | Shelf Life: | 1-2 Years |
| High Light: | 50T Animal MiRNA Isolation Kits,99.9% Animal MiRNA Isolation Kits,RE-0101T RNA Isolation Kits |
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Room temperature operation Animal miRNA Isolation kits fast and high RNA yield
Description
Animal miRNA Isolation Kit use the spin column and formula developed by our company, which can efficiently extract small fragments of RNA such as 20-200nt miRNA, siRNA, snRNA from various animal tissues and cells. The kit provides DNA-Cleaning Column, which can easily separate the supernatant from the tissue lysate and remove DNA and long RNA from the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA with a unique formula , Can process a large number of samples at the same time. The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miRW1, Buffer miRW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ions, and organic compound pollution.
Kit components
| Animal miRNA Isolation Kits | ||||
| Kit contents | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100 T | 200 T | |
| Buffer miRL* | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| RNA-only Column | 5 sets | 50 sets | 100 sets | 200 sets |
| DNA-Cleaning Column | 5 sets | 50 sets | 100 sets | 200 sets |
| IFU | 1 piece | 1 piece | 1 piece | 1 piece |
Features&advantages
| Room temperature (15-25°C) operation | without ice bath and low-temperature centrifugation |
| RNase-Free | no need to worry about RNA degradation. |
| DNA-Cleaning Column specifically binds DNA | the Animal miRNA Isolation kit can remove genomic DNA contamination without adding additional DNase. |
| High RNA yield | RNA-only Column and unique formula can efficiently purify RNA. |
| Fast speed | The operation is simple and can be completed within 30 minutes. |
| Safety | No organic reagent extraction is required. |
| High quality | The extracted RNA fragments are of high purity, free of protein and other impurities pollution, and can meet the requirements of downstream. |
| Applications | Various experimental applications |
Kit application
Animal MiRNA Isolation Kit is suitable for the extraction and purification of miRNA from a variety of fresh or frozen animal tissues or cells.
The miRNA purified by Animal miRNA isolation kit can be used in various downstream molecular experiments, such as: RT-PCR, Real Time PCR, Northern Blot, chip analysis, molecular cloning, etc.
It is recommended to use RNase-Free ddH2O to elute RNA for immediate use in downstream experiments or store at -80°C. Under the storage condition of -80℃, RNA can be stored for one year.
Storage and Shelf life
Animal MiRNA Isolation Kit can be stored for 24 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C. Buffer miRL can be placed at 4°C for 1 month after adding β-mercaptoethanol (it is recommended to add it now).
Kit components
◆ Buffer miRL: Provides the environment for grinding and lysis of animal tissues and the column environment.
◆ Buffer miRW1: Remove impurities such as protein and DNA in RNA.
◆ Buffer miRW2: Remove residual salt ions in RNA.
◆ RNase-Free ddH2O: to elute the total RNA on the purification column membrane.
◆ DNA-Cleaning Column: specifically adsorb DNA and long fragment RNA in tissue lysate, and filter
◆ Remove the solid impurities in the cracked product.
RNA-only Column: specifically adsorb small fragments of RNA.
Product INFO
| Model | Spin-column type | Purification components | Foregene Spin column, reagent |
| Flux | 1-24 samples | Preparation time | ~30min(24 samples) |
| Centrifuge | Table centrifuge |
Tissue enzymolysis separation |
centrifugal separation |
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Purification column RNA load carrying capacity |
80μg |
Liquid volume of spin column |
800μl |
| Elution volume | 50-200μl |
Tissue sample processing volume |
Tissue≤30mg Cell≤5×106 |
According to FOREGENE’s Total Quality Management System, each batch of Animal miRNA isolation kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
DNA-Cleaning Column Characters
| Mechanism | Specific adsorption of DNA, long RNA fragments above 200 nt |
| Function | Removal of DNA and long fragment RNA contamination, filtration separation and lysis |
| Maximum loading volume | 800μl |
| Maximum binding capacity | 20μg |
| Maximum loading volume | 800μl |
| RNA size distribution | 20nt≤RNA≤200nt |
| Minimum elution volume | 30μl |
| Selection of samples | Animal tissue or animal cell |
| Maximum amount of starting material | 30mg animal tissue or 5×106 cells |
1*: The minimum elution system of 30μl is a reasonable recommended volume considering the RNA recovery rate and concentration. If you want to increase the yield of RNA, you can increase the volume of the eluate; if you want to increase the concentration of the purified RNA, reduce the volume of the eluate appropriately, such as using a 15μl elution system, while sacrificing part of the RNA yield. In order to get a higher concentration of RNA.
2*: For larger sample volumes, please use multiple RNA-Only Columns for RNA purification.
RNA extraction yield and purity
The Animal miRNA Isolation Kit can be used to purify miRNA from a variety of tissues and cells. The yield is related to the tissue sample itself, the initial amount of the sample, the freshness of the sample, the storage time of the sample, and the operation. The purified RNA OD260/280=1.8-2.1.
RNA integrity
The figure below shows the RNA electrophoresis image obtained by processing animal tissue samples with Foregene Animal miRNA Isolation Kit.
Work Flow
Notes: (Please read the notes carefully before using the kit)
◆ All experimental steps are performed at room temperature (15-25°C) (including centrifugation). Do not use ice bath and low-temperature (4°C) centrifugation.
◆ The sample should avoid repeated freezing and thawing, otherwise the extracted miRNA will be degraded and the extraction amount will also decrease.
◆ The amount of fresh tissue should not exceed 30mg, otherwise it will affect the yield and purity of miRNA.
◆ Before using the kit, add β-mercaptoethanol to Buffer miRL. Add 10μl of β-mercaptoethanol per 1ml of Buffer miRL. It is recommended to prepare it for immediate use. After adding β-mercaptoethanol, Buffer miRL can be stored at 4°C for 1 month.
◆ Before using the kit, add absolute ethanol to Buffer miRW2. Please refer to the label on the reagent bottle for the added amount.
◆ See the table below for the addition amount of anhydrous ethanol in the kits of different specifications:
| Product specifications | Amount of anhydrous ethanol added |
| RE-0101T | 6ml |
| RE-01011 | 60ml |
| RE-01012 | 120ml |
| RE-01014 | 240ml |
◆ RNA yield and quality are related to the elution volume and the amount of tissue sample. It is recommended to use 30 mg of tissue per 500 μl of Buffer miRL.
◆ Elution volume: The elution volume should not be less than 30μl, otherwise it will affect the RNA recovery efficiency.
◆ Please check the Buffer miRL and Buffer miRW1 in the kit for crystals. If crystals are precipitated after storage at low temperature, you can put the Buffer at room temperature or 37°C for a period of time, dissolve the crystals and mix well before use.
-This product is for scientific research use only, please do not use it for medicine, clinical medicine, food and cosmetics.
- When using the kit, please wear lab coats, disposable latex gloves, disposable masks, etc. to protect yourself; and avoid artificially introduced RNase contamination to the greatest extent possible.
- Buffer miRL contains chaotropic salts: denaturant, irritant.
- Buffer miRW2 contains ethanol: Flammable.
Contact Person: Maggie
Tel: +8615281067355
