Place of Origin: | China |
Brand Name: | FOREGENE |
Certification: | ISO CE |
Model Number: | RE-02012 |
Minimum Order Quantity: | 1 kits |
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Price: | Negotiable |
Packaging Details: | Packing with safe trans carton |
Delivery Time: | 5-8 days |
Payment Terms: | L/C, T/T, Western Union |
Supply Ability: | 100000 kits per month |
Product Name: | Viral RNA Isolation Kits | Packing: | 100 Tests Per Kits |
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Application: | RNA Isolation Kit For Plasma Serum Cell-Free Body Fluid And Cell Culture Supernat | OEM: | Accept |
MOQ: | 1 Kit | Type: | Spin Column Technology |
High Light: | Serum Plasma RNA Isolation Kits,RNase Free RNA Isolation Kits,High RNA Yield RNA Extraction Kits |
High RNA yield Serum plasma RNA Isolation kits for RNA extraction and purification
Description:
The Serum(Plasma) miRNA Isolation kit uses the spin column and formula developed by our company, which can efficiently extract small fragments of miRNA less than 100nt from serum and plasma. The kit provides DNA-Cleaning Column, which can easily separate the supernatant and tissue lysate and adsorb and remove the DNA in the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA, and with a unique formula, it can be used at the same time. Process a large number of samples.
The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miBRW1 and Buffer miBRW2 buffer washing system make the RNA obtained without protein, DNA, ion, and organic compound pollution.
Specifications:
50 Preps, 200 Preps
Kit components:
Serum/Plasma miRNA Isolation Kit | ||||
Kit contents | RE-0111T | RE-01111 | RE-01112 | RE-01114 |
5 T | 50 T | 100 T | 200 T | |
Buffer miBRL1* | 2ml | 20ml | 40ml | 80ml |
Buffer miBRL2* | 1.2ml | 12ml | 24ml | 48ml |
Buffer miBRW1 | 2ml | 20ml | 40ml | 80ml |
Buffer miBRW2 | 2.4ml | 24ml | 48ml | 96ml |
RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
RNA-only Column | 5 pieces | 50 pieces | 100 pieces | 200 pieces |
DNA-Cleaning Column | 5 pieces | 50 pieces | 100 pieces | 200 pieces |
IFU | 1 piece | 1 piece | 1 piece | 1 piece |
*: Buffer miBRL1 and miBRL2 contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.
Features&advantages:
◆ Full set kit RNase-Free, no need to worry about RNA degradation.
◆ DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
◆ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
◆ Fast speed: The operation is simple and can be completed within 30 minutes.
◆ Safety: No organic reagent extraction is required.
◆ High quality: The extracted RNA fragments have high purity, free of protein and other impurities, and can meet various downstream experimental applications.
Product INFO
Model | Spin-column type | Purification components | Foregene Spin column, reagent |
Flux | 1-24 samples | Preparation time | ~30min(24 samples) |
Centrifuge | Table centrifuge |
Tissue lysate isolation |
centrifugal isolation |
Purification column RNA load carrying capacity |
20μg |
Liquid volume of spin column |
800μl |
Elution volume | 30-50μl |
Tissue sample processing volume |
≤200μl |
Kit application:
The Serum(Plasma) miRNA Isolation kit is suitable for extraction and purification of miRNA from animal and human serum and plasma.
miRNA Application
The miRNA purified by Serum/Plasma miRNA Isolation Kit can be used in various downstream molecular experiments, such as: RT-PCR, Real Time RT-PCR, Northern Blot, chip analysis, etc.
RNA storage
It is recommended to use RNase-Free ddH2O to elute RNA for immediate use in downstream experiments or store at -80℃. RNA can be stored for one year under -80℃ storage condition.
Quality Control
According to FOREGENE's Total Quality Management System, each batch of Serum/Plasma miRNA Isolation Kit is strictly tested for multiple times to ensure the reliability and stability of each batch of kit quality .
Work Flow:
Using Serum/Plasma miRNA Isolation Kit can obtain the RNA from serum and plasma purification, and its yield is related to the sample itself, the initial amount of the sample, the freshness of the sample, the storage time of the sample, and the operation. The purified RNA OD260/280=1.8-2.1.
Storage conditions
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C). If it needs to be stored for a longer time, please stored at 2-8°C.
Contact Person: Maggie
Tel: +8615281067355