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Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

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Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy

Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy

  • Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy
  • Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy
  • Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy
Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy
Product Details:
Place of Origin: China
Brand Name: Foreasy
Certification: ISO
Model Number: IM-02011
Payment & Shipping Terms:
Minimum Order Quantity: 200 KU
Price: 900 usd/200 KU
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 1000000 ku per month
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Detailed Product Description
Brand: Foreasy Specifications: 1ml X 5,200 KU
Type: MMLV Reverse Transcriptase Applicable Samples: M-MLV For Reverse Transcriptase
Product Name: M-MLV Reverse Transcriptase MOQ: 200 KU
High Light:

MMLV Reverse Transcriptase

,

1ml MMLV Reverse Transcriptase

,

MMLV Reverse Transcriptase RT Enzyme

Foreasy M-MLV Reverse Transcriptase

Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy For Sale

Product Introduction

Foreasy M-MLV Reverse Transcriptase is a new reverse transcriptase expressed in E. coli engineered bacteria using genetic recombination technology. It is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. It has no RNase H activity, strong stability, strong RNA affinity, and high detection sensitivity.

 

Foreasy New Generation Of M-MLV Reverse Transcriptase RT Enzyme Foreasy 0

Product components:

Component IM-02011 IM-02012 IM-02013

Foreasy Reverse Transcriptase

(200 U/μL)

200 KU (1 mL) 2000 KU (10 mL) 5000 KU (25 mL)

5× Foreasy

RT Buffer

1 mL ×5 10 mL ×5 10 mL ×12

 

Type

Cat No. Type
IM-02011 200 KU 200 U/μL
IM-02012 2,000 KU 200 U/μL
IM-02013 5,000 KU 200 U/μL

 

Storage

Store at -20±5℃ for 2 years or at -80℃ for long-term storage.

 

Application

u Synthesis of the first strand of cDNA for RT-PCR and real-time RT-PCR

u cDNA synthesis for cloning and expression.

u Generation of labeled cDNA probes for microarrays.

u Primer extension RNA analysis

 

Unit definition


One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of [ 3H] dTTP in 10 min at 37°C using poly(A)oligo(dT)25 as template-primer.

 

Unit reaction conditions


50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)25, 0.1 mg/mL BSA, and enzyme in 50 µL for 10 min at 37°C.

 

Operation :

synthesize the first strand cDNA (20 μL reaction system)

1. RT system structure (the following steps should be performed on ice).

1.1 After defrosting each compoment, add it to the reaction tube in the order of following Table 1

 

 

Table 1:

RT system add content

 

Volume

Final concentration

 

Template RNA X µL Total RNA:<5 μg / mRNA:<0.5 μg
Random Primer 0.5 µg 100 pmol
Or Oligo(dT)18 Primer 0.2 µg 100 pmol
Or Specific Primer 15-20 pmol 15-20 pmol
RNase-Free ddH2O to 12.5 µL

1.2 After the system is prepared, mix gently and centrifuge briefly, then perform RT reaction according to the below in Table 2

Table 2

 

Step Temperature Time Content
1 65℃ 5 min Denaturation
Cool quickly on ice after finishing the reaction
 
  • Adding the Reactant in the order as the following table 3 after cool on the ice

 

Table:3:

RT system add content

 

Volume

Final concentration

 

5× Foreasy RT Buffer 4 µL
Foreasy RNase Inhibitor (40 U/µL) 0.5 µL 1 U/μL
dNTP Mix (10 mM each) 2 µL 1 mM
Foreasy Reverse Transcriptase (200 U/µL) 1 µL 10 U/μL

 

1.4 After the system prepared, mix gently and centrifuge briefly, then perform RT reaction conditions in following Table 4

Table 4:

Step Temperature Time Content
1 42℃ 50 min cDNAsynthesis
2 70℃ 10min Inactivated reverse transcriptase
3 4℃ N/A Place it at 4°C for later use or store at -20°C after reaction

Note: When using Random Primer, the reaction should be preheated at 25°C for 10 minutes before the first step reaction.

The above procedure is for reference only, and the actual reaction conditions vary depending on the structure of the template, primers, etc.

After the reaction is completed, the reaction product is placed on ice for subsequent experiments directly; for long-term storage, please store it at -20°C or -80°C.

Contact Details
Foregene Co., Ltd.

Contact Person: David

Tel: +8616602829025

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