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Foregene Co., Ltd.
Foregene Co., Ltd.

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RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research

RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research

  • RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research
  • RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research
  • RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research
RT Easy I 2×High Efficiency PCR Reaction Premix System For Lab Research
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO CE
Model Number: RT-01022
Payment & Shipping Terms:
Minimum Order Quantity: 10 kits
Price: USD 220 per kit
Packaging Details: Packing with safe trans carton
Delivery Time: 7-10 working days
Payment Terms: T/T, Western Union, L/C, D/P
Supply Ability: 10000 kits per month
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Detailed Product Description
Product Name: Pcr Premix System Packing: 100 Reactions Per Kit
Certificates: ISO Applification: For First-strand CDNA Synthesis
MOQ: 10 Kits Leading Time: 10 Working Days
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PCR Reaction Premix System


RT Easy PCR Reaction Premix System


ISO Lab PCR Kits

RT Easy I 2×High-efficiency PCR reaction premix system for lab research use


RT Easy I Master Premix For First-Strand CDNA Synthesis Fast And Highly Sensitive Reverse Transcription System


FOREGENE’s 2× RT EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.

This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.


The RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT EasyTM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.

RT Easy I Master Premix for first-strand cDNA synthesis Fast and highly sensitive reverse transcription system




25T, 100T


Kit components:



Master Premix for first-strand cDNA synthesis

Kit contents RT-01011 RT-01012
25 times (20μl system) 100 times (20μl system)
2× RT EasyTM Mix 0.25ml 1ml
Random Primer (50μM) 50μl 200μl
Oligo(dT)18 Primer (50μM) 50μl 200μl
RNase-Free ddH2O 1.7ml 1.7ml
Instructions Manual 1 piece 1 piece



u Efficient reverse transcription system, it only takes 25 minutes to complete the synthesis of the first strand cDNA.

u High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.

u The reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.

u Even impure RNA samples (alcohol up to 45%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.

Kit component information

u 2× RT EasyTM Mix: Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, reaction buffer, optimizer and stabilizer, etc.

u Random Primer (50μM): 6 oligonucleotide random primers with a concentration of 50μM.

u Oligo(dT)18 Primer(50μM): a primer with 18 dT,the concentration is 50μM


Kit application

u Conventional RT-PCR

u Synthesis of cDNA for cloning and expression research

u Analysis of the primer extension method of the transcription initiation site

u RACE (rapid amplification of cDNA ends)

u Linear RNA amplification

u Chip mark


Precautions: (Please read the precautions carefully before using the kit)

u For templates, it is recommended to use RNA extracted from fresh samples or stored at -80 (RNA should avoid repeated freezing and thawing).

u In order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn.

u Before use, put the 2×RT EasyTM Mix on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and the specificity of PCR amplification.

u 2× RT EasyTM Mix does not add reverse transcription primers, you need to add appropriate concentrations of Random Primer or Oligo(dT)18 Primer or specific primers according to the experimental requirements.



Transport and storage conditions

1.Transportation conditions

The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.


2. Storage conditions

RT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

Contact Details
Foregene Co., Ltd.

Contact Person: David

Tel: +8616602829025

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