Send Message
Tel:
Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

CHINA FOREGENE,WORLD'S FOREGENE!

Home ProductsPCR Kits

Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction

Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction

  • Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction
  • Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction
  • Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction
Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO CE
Model Number: TP-01411/01412/01413
Payment & Shipping Terms:
Minimum Order Quantity: 1 Kit
Price: Negotiable
Packaging Details: Packing with safe trans carton
Delivery Time: 5-8 days
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100000 kits per month
Contact Now
Detailed Product Description
Product Name: Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction Type: Direct PCR Kit
Package: 200T, 500T, 2000T Feature: Higher Specificity And Stronger Tolerance To PCR Reaction Inhibitors
Application: For Freshwater Fish Tissues Samples PCR Test Oem: Accept
High Light:

Oem PCR Kits

,

Freshwater Fish Direct PCR Kits

Higher specificity PCR System Zebra Fish Direct PCR Kits for direct PCR reaction

 

 

Description:

 

Zebra Fish Direct PCR Kit uses a unique lysis buffer system to quickly release genomic DNA from zebrafish and other freshwater fish tissues, tail fins or fish eggs samples for PCR reactions, so it is particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2×PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer. Used in conjunction with the lysis buffer can quickly and easily detect samples, and has the characteristics of high sensitivity, strong specificity, and good stability.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

 

Kit components:

 

Zebra Fish Direct PCR Kit
Kit contents TP-0141T TP-01411 TP-01412 TP-01413
50T 200T 500T 2000T
Part I Buffer FP 5ml 20ml 50ml 100ml×2
Foregene Protease 220μl 880μl 1.1ml×2 8.8ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Part II 2× PCR EasyTM Mix 500μl 1ml×2 1.7ml×3 1.7ml×12
IFU 1piece 1piece 1piece 1piece
 

 

 

Features&advantages:

 

-No time-consuming and expensive DNA purification is required.

-The sample demand is small, as little as 1mg fish caudal fin or 10 fish eggs can be tested.

-No special treatments such as grinding and crushing are required, and the operation is simple.

-Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

 

 

Kit parameters:

 

Application: Zebra fish and other fresh water fish.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

 

 

Work Flow:

 

Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction 0

 

Electropherogram:

 

Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction 1

 

Storage and Shelf life:

 

Part I of this kit should be stored at 2-8℃.

The reagent Buffer FP can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

Higher Specificity PCR System Zebra Fish Direct PCR Kits For Direct PCR Reaction 2

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
Other Products
Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
Mobile Site Privacy Policy | China Good Quality PCR Kits Supplier. © 2022 - 2023 foregenebio.com. All Rights Reserved.