Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 |
| Place of Origin: | China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | TP-01331/01333 |
| Minimum Order Quantity: | 1 kit |
|---|---|
| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 200 ×20μl Rxns,2000×20μl Rxns |
|---|---|---|---|
| Type: | Mouse Tail Direct PCR | Application: | Extraction And Purification Of Genomic DNA From Fresh Or Frozen Large And Mouse Tails |
| Product Name: | Lab Reagent Higher Specificity Mouse Tail Direct PCR Kits Without Prior DNA Purification 65℃ Lysis | Key Word: | Direct PCR; DNA-Only Column |
Lab Reagent higher specificity Mouse Tail Direct PCR Kits without prior DNA purification 65℃ lysis
Product Descprition
This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.
The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.
2× M1-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.
Kit contents:
| Mouse Tail Direct PCR Kit | |||||
| Kit contents | TP-0133T | TP-01331 | TP-01332 | TP-01333 | |
| 50T | 200T | 500T | 2000T | ||
| Part I | Buffer MP | 5ml | 20ml | 50ml | 100ml×2 |
| Foregene Protease Plus | 220μl | 880μl | 1.1ml×2 | 8.8ml | |
| 6× DNA Loading Buffer | 1.5ml | 1.5ml | 1.5ml | 1.5ml×4 | |
| Part II | 2× M1-PCR EasyTM Mix | 500μl | 1ml×2 | 1.7ml×3 | 1.7ml×12 |
| IFU | 1piece | 1piece | 1piece | 1piece | |
Features&advantages:
-No time-consuming and expensive DNA purification is required.
-The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.
-No special treatments such as grinding and crushing are required, and the operation is simple.
-The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
Kit Application:
Mouse tail, mouse ears, muscle and other tissues.
DNA released from sample lysis: only used as a PCR template.
The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.
Storage and Shelf life:
Part I of this kit should be stored at 2-8℃.
-The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.
-Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.
-Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.
Part II of this kit should be stored at -20℃ .
-Reagent 2×M1 PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Contact Person: Maggie
Tel: +8615281067355
