Send Message
Tel:
Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

CHINA FOREGENE,WORLD'S FOREGENE!

Home ProductsDirect PCR Kits

Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis

Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis

  • Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis
  • Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis
  • Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis
  • Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis
Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis
Product Details:
Place of Origin: China
Brand Name: Foregene
Certification: CE, ISO
Model Number: TP-01121/01123
Payment & Shipping Terms:
Minimum Order Quantity: 1 kit
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
Contact Now
Detailed Product Description
Factory: Foregene Specifications: 200*20μl Rxns,2000*20μl Rxns
Type: Direct PCR Master Mix-UNG Application: A Variety Of Animal Tissues
Product Name: Optimized Anti-pollution PCR System Animal Tissue Direct PCR Kits UNG For Identification Of Transgenosis Key Words: Direct PCR; Animal Tissue

 

Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis

 

 

Product Descprition

 

This kit uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as template for PCR reaction.

2×PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2×PCR EasyTM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of PCR products contamination during large-scale genetic testing.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis 0

Product components:

 

Animal Tissue Direct PCR Kit-UNG
Kit contents TP-0112T TP-01121 TP-01122 TP-01123
50T 200T 500T 2000T
Part I Buffer AL 5ml 20ml 50ml 100ml×2
Foregene Protease 220μl 880μl 1.1ml×2 8.8ml
6× DNA Loading Buffer 1.5ml 1.5ml 1.5ml 1.5ml×4
Part II 2× PCR EasyTM Mix(UNG) 500μl 1ml×2 1.7ml×3 1.7ml×12
IFU 1piece 1piece 1piece 1piece
 

 

Features&advantages:

  • No time-consuming and expensive DNA purification
  • The sample demand is small, as little as 5mg animal tissue can be tested.
  • No special treatment such as grinding and crushing is required, and the operation is simple.
  • Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.
  • Anti-pollution PCR system 2×PCR EasyTM Mix (UNG) effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.

 

Application:

 

- Application: a variety of animal tissues.

- DNA released from sample lysis: only used as a PCR template.

- The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

 

Product quality control:

 

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Animal Tissue Direct PCR kits-UNG is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

 

Storage and Shelf life:

 

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

 Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

 Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

 Reagent 2×PCR EasyTM Mix(UNG), if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

 

Precautions:(Please read the precautions carefully before using the kit)

 

- Pay attention to the cleanliness of laboratory equipment and the operation method of the experiment to avoid cross-contamination between samples.

- Please try to use freshly collected animal tissue samples for experiments. If the tissue samples are stored for a long time, please avoid repeated freezing and thawing of the samples.

- If there is precipitation in Buffer AL, it can be placed at 37℃ until the precipitation disappears, and the solution is shaken before use.

- Foregene Protease has a unique formula, please store at 4°C, never at -20°C.

- 2× PCR EasyTM Mix (UNG) should avoid repeated freezing and thawing, otherwise it will affect the PCR efficiency.

- If the ambient temperature is too high, the 2× PCR EasyTM Mix (UNG) may become cloudy. It can be placed on ice for 1-2 minutes. After the solution is clear, invert and mix for 3-5 times before use.

- When electrophoresis detection, do not use Loading Buffer containing SDS, otherwise a large group of trailing bright bands will appear in the swimming lane during electrophoresis, which will affect the experimental results.

Optimized Anti-pollution PCR system Animal Tissue Direct PCR Kits UNG for identification of transgenosis 1

 

 

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
Other Products
Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
Mobile Site Privacy Policy | China Good Quality PCR Kits Supplier. © 2022 - 2024 foregenebio.com. All Rights Reserved.