PCR Kits Premix System Containing Taq Enzyme High Efficiency

Product Details:

Place of Origin:	China
Brand Name:	FOREGNE
Certification:	ISO CE
Model Number:	PH-01015-B

Payment & Shipping Terms:

Minimum Order Quantity:	10 ml
Price:	Negotiable
Packaging Details:	Packing with safe trans carton
Delivery Time:	5-8 days
Payment Terms:	L/C, T/T, Western Union
Supply Ability:	100000 ml per month

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Detailed Product Description

Features:	Higher Fidelity,Faster Amplification Speed	Product Name:	PCR Kits Premix System Containing Taq Enzyme High Efficiency
Type:	PCR Reaction Premix System	MOQ:	10ml
OEM:	Accept	Application:	For PCR Reaction
High Light:	High Efficiency PCR Reaction Premix System , 2× PCR HeroTM Mix

PCR kits premix system containing Taq enzyme high efficiency

PCR Kits Premix System Containing Taq Enzyme High Efficiency 0

Description:

This kit includes Foregene's unique new generation of Taq—Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

Specifications:

5ml, 10ml, 40ml

Kit(2× PCR HeroTM Mix) components:

0.1U Taq Hero/μl

400μM dNTPs

20mM Tris-HCl(pH8.8)

100mM KCl

5mM MgCl2

Other stabilizers and enhancers

DNA Loading Buffer

Features&advantages:

Higher fidelity	6 times that of ordinary Taq enzyme;
Faster amplification speed	the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;
More template adaptability	it can efficiently amplify various complex DNA templates with high GC content and difficult to amplify;
Higher Amplification efficiency	the number of amplification cycles is lower than that of ordinary Taq enzyme;
Stronger Environmental tolerance	placed at 37°C for a week, maintaining more than 90% activity;
Activity	It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity.

Practical examples

1.Reaction system

2× PCR HeroTM Mix(dye)	10μl
Template DNA	xμl
Primer 1(10μM)	0.4μl
Primer 2(10μM)	0.4μl
ddH2O	Fill to 20μl
Total volume	20µl

2.Reaction conditions

Temperature	Time	Cycles
94°C	3min	1
94°C	10sec	25-40
55-65°C	10sec
72°C	≥10sec (5-20sec/kb)1*
72°C	5min	1

1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.

Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.

Kit application:

PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail

Storage and Shelf life:

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

Instructions

PCR Hero^TM(With Dye) is convenient and quick to use, and can avoid the pollution during the PCR operation and the experimental error caused by multiple preparations of the reaction system to the greatest extent. Use only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2× PCR Hero^TM Mix(dye)) of 2× PCR Hero^TMMix(dye), add template and primers, and add deionized water to make up Volume, make the PCR Mix concentration in the reaction system 1×, then the reaction can be carried out.

Practical examples

1.Reaction system