Plant Total RNA Isolation Kit For Plant Low In Polysaccharide And Polyphenol RNA Isolation Kits Spin Column

Plant Total RNA Isolation Kit For Plant Low In Polysaccharide And Polyphenol RNA Isolation kits spin column

Description:

The Plant Total RNA Isolation kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.

Specifications:

50 Preps, 200 Preps

Kit components:

*: Please wear gloves and take protective measures during the operation as PRL1 and buffer PRW1 contain irritating chaotropic salts.

Product information

Features&advantages:

-Particularly suitable for the purification of RNA from plant samples with low polysaccharides and polyphenols content.

-No need to worry about RNA degradation. The whole system is RNase-Free

-Effectively remove DNA using DNA-Cleaning Column

-Remove DNA without adding DNase

-Simple—all operations are completed at room temperature

-Fast—operation can be completed in 30 minutes

-Safe—no organic reagent used

-High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

Kit application:

This Plant RNA isolation kits is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

Kit component information

Buffer PRL1:Provides the environment required for animal tissue grinding and cracking.

Buffer PRL2 :Provides a specific upper column environment for RNA.

Buffer PRW1 :Removes proteins, DNA and other impurities from RNA.

Buffer PRW2:Remove residual salt ions from the RNA.

RNase-Free ddH2O:Elute total RNA on the purified column membrane.

DNA-Cleaning Column :Specifically adsorbs DNA in tissue lysates, and filters to remove solid impurities in the lysates.

RNA-only Column :Specific adsorption of the total RNA through the DNA-Cleaning Column filtrate.

Work Flow:

DNA-Cleaning Column specifications

RNA-Only Column specifications

1*:The minimum elution system of 50 μl is a reasonably recommended volume given taking into account RNA recovery and concentration. If in order to improve the yield of RNA, can appropriately increase the elution volume ; if in order to increase the concentration of the purified RNA,in the premise of a portion of the RNA yield sacrificed, the elution volume should be reduced appropriately, such as the use of a 30 μl elution system in order to obtain a higher concentration of RNA.

2*: For larger sample sizes, use multiple RNA-Only Columns for RNA purification.

RNA completeness

The quality of the RNA can be analyzed under ultraviolet light after denaturing agarose gel electrophoresis, ethidium bromide staining. On the gel, the band pattern of the ribosomal RNA should be obvious and clear; the luminance ratio of 28S RNA to 18S RNA should be about 2:1. 4 or more rRNAs are visible in plant leaves due to the large amount of chloroplast RNA. If ribosomal RNA or specific sample bands in any of the lanes appear inconspicuous, unclear, diffuse into small fragments of RNA, or disappear, it is possible that the sample has undergone RNA degradation prior to processing or that caused RNA degradation during purification.

The figure below shows the RNA electrophoresis map obtained by Plant Total RNA Isolation Kit processing plant samples.

RNA elution recovery efficiency

After many rigorous experiments, we found that the elution recovery efficiency of purified RNA is related to the elution system and the number of elutions, and the specific data are shown in the chart below. Based on the elution efficiency given in the figure, the user can choose the appropriate elution system according to their experimental needs in order to obtain a considerable yield of RNA and the desired RNA concentration.

Note: The elution recovery efficiency in the above figure is for reference only, and the specific recovery elution yield is subject to the final experimental results, which may be possible

There will be some discrepancies with the data in the above figure, and a deviation of 10-20% is normal.

Storage and Shelf life:

The Plant RNA Isolation kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PRL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).