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Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

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200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul

200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul

  • 200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul
200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul
Product Details:
Place of Origin: Sichuan, China
Brand Name: Foregene
Certification: CE, ISO
Model Number: 5T, 50T, 100T, 250T
Payment & Shipping Terms:
Minimum Order Quantity: 100kits
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Product Name: Serum(Plasma) MiRNA Isolation Kits
CAT NO.: RE-0111T, RE-01111, RE-01112, RE-01114 Type: RNA-Only Column
Application: Animal And Human Serum And Plasma Operation Temperature: Room Temperature
High Light:

Plasma miRNA Isolation Kit

,

Spin Column Type RNA Isolation Kits

 

RNA Isolation kits 200 pieces RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul
 
200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul 0
 
Description
 

This kit uses the spin column and formula developed by our company, which can efficiently extract small fragments of miRNA less than 100nt from serum and plasma. The kit provides DNA-Cleaning Column, which can easily separate the supernatant and tissue lysate and adsorb and remove the DNA in the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA, and with a unique formula, it can be used at the same time. Process a large number of samples.
The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miBRW1 and Buffer miBRW2 buffer washing system make the RNA obtained without protein, DNA, ion, and organic compound pollution.
 
 
 
Kit conponents
 

Serum/Plasma miRNA Isolation Kit

Kit contentsRE-0111TRE-01111RE-01112RE-01114
5 T50 T100 T200 T
Buffer miBRL1*2ml20ml40ml80ml
Buffer miBRL2*1.2ml12ml24ml48ml
Buffer miBRW12ml20ml40ml80ml
Buffer miBRW22.4ml24ml48ml96ml
RNase-Free ddH2O1.7ml10ml20ml40ml
RNA-only Column5 pieces50 pieces100 pieces200 pieces
DNA-Cleaning Column5 pieces50 pieces100 pieces200 pieces
IFU1 piece1 piece1 piece1 piece

 

Product INFO

ModelSpin-column typePurification componentsForegene Spin column, reagent
Flux1-24 samplesPreparation time~30min(24 samples)
CentrifugeTable centrifuge

Tissue lysate

isolation

centrifugal isolation

Purification column

RNA load carrying capacity

20μg

Liquid volume of

spin column

800μl
Elution volume30-50μl

Tissue sample

processing volume

≤200μl

 
Features&advantages

 
- Full set kit RNase-Free, no need to worry about RNA degradation.

- DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.

- High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.

- Fast speed: The operation is simple and can be completed within 30 minutes.

- Safety: No organic reagent extraction is required.
- High quality: The extracted RNA fragments have high purity, free of protein and other impurities, and can meet various downstream experimental applications.
 

Kit application
 
It is suitable for extraction and purification of miRNA from animal and human serum and plasma.

 

Storage and Shelf life
 
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C). If it needs to be stored for a longer time, please stored at 2-8°C.
 

Kit components

◆ Buffer miBRL1& miBRL2: Provides the environment required for serum/plasma lysis and the column passing.
◆ Buffer miBRW1: Remove impurities such as protein and DNA in RNA.
◆ Buffer miBRW2: Remove residual salt ions in RNA.
◆ RNase-Free ddH2O: Elute the miRNA on the purification column membrane.
◆ DNA-Cleaning Column: Specifically adsorb the DNA in the lysate, and filter to remove solid impurities in the lysate.
◆ RNA-only Column: Specifically adsorb the RNA in the filtrate passing through the DNA-Cleaning Column.
 
miRNA Application

The miRNA purified by Serum/Plasma miRNA Isolation Kit can be used in various downstream molecular experiments, such as: RT-PCR, Real Time RT-PCR, Northern Blot, chip analysis, etc.

 

RNA storage

It is recommended to use RNase-Free ddH2O to elute RNA for immediate use in downstream experiments or store at -80℃. RNA can be stored for one year under -80℃ storage condition.

 

Quality Control

According to FOREGENE's Total Quality Management System, each batch of Serum/Plasma miRNA Isolation Kit is strictly tested for multiple times to ensure the reliability and stability of each batch of kit quality .

 

RNA extraction yield and purity

Using Serum/Plasma miRNA Isolation Kit can obtain the RNA from serum and plasma purification, and its yield is related to the sample itself, the initial amount of the sample, the freshness of the sample, the storage time of the sample, and the operation. The purified RNA OD260/280=1.8-2.1.

 
Work Flow
200 Pieces RNA Isolation Kits RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul 1

Contact Details
Foregene Co., Ltd.

Contact Person: Maggie

Tel: +8615281067355

Send your inquiry directly to us
Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
Tel:86-028-86050301
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