Place of Origin: | Sichuan, China |
Brand Name: | Foregene |
Certification: | CE, ISO |
Model Number: | 5T, 50T, 100T, 250T |
Minimum Order Quantity: | 100kits |
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Price: | Negotiable |
Packaging Details: | craft paper bag |
Delivery Time: | 5-8 working days |
Payment Terms: | L/C, D/A, D/P, T/T |
Supply Ability: | 100000kits per month |
Factory: | Foregene | Product Name: | Serum(Plasma) MiRNA Isolation Kits |
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CAT NO.: | RE-0111T, RE-01111, RE-01112, RE-01114 | Type: | RNA-Only Column |
Application: | Animal And Human Serum And Plasma | Operation Temperature: | Room Temperature |
High Light: | Plasma miRNA Isolation Kit,Spin Column Type RNA Isolation Kits |
RNA Isolation kits 200 pieces RNase Free RNA Only Column High RNA MiRNA Isolation Kits From Serum 200ul
Description
This kit uses the spin column and formula developed by our company, which can efficiently extract small fragments of miRNA less than 100nt from serum and plasma. The kit provides DNA-Cleaning Column, which can easily separate the supernatant and tissue lysate and adsorb and remove the DNA in the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA, and with a unique formula, it can be used at the same time. Process a large number of samples.
The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miBRW1 and Buffer miBRW2 buffer washing system make the RNA obtained without protein, DNA, ion, and organic compound pollution.
Kit conponents
Serum/Plasma miRNA Isolation Kit | |||||||||||||||||||||||||||||||||||||||||||||||||
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Model | Spin-column type | Purification components | Foregene Spin column, reagent |
Flux | 1-24 samples | Preparation time | ~30min(24 samples) |
Centrifuge | Table centrifuge | Tissue lysate isolation | centrifugal isolation |
Purification column RNA load carrying capacity | 20μg | Liquid volume of spin column | 800μl |
Elution volume | 30-50μl | Tissue sample processing volume | ≤200μl |
Features&advantages
- Full set kit RNase-Free, no need to worry about RNA degradation.
- DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
- High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
- Fast speed: The operation is simple and can be completed within 30 minutes.
- Safety: No organic reagent extraction is required.
- High quality: The extracted RNA fragments have high purity, free of protein and other impurities, and can meet various downstream experimental applications.
Kit application
It is suitable for extraction and purification of miRNA from animal and human serum and plasma.
Storage and Shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C). If it needs to be stored for a longer time, please stored at 2-8°C.
◆ Buffer miBRL1& miBRL2: Provides the environment required for serum/plasma lysis and the column passing.
◆ Buffer miBRW1: Remove impurities such as protein and DNA in RNA.
◆ Buffer miBRW2: Remove residual salt ions in RNA.
◆ RNase-Free ddH2O: Elute the miRNA on the purification column membrane.
◆ DNA-Cleaning Column: Specifically adsorb the DNA in the lysate, and filter to remove solid impurities in the lysate.
◆ RNA-only Column: Specifically adsorb the RNA in the filtrate passing through the DNA-Cleaning Column.
miRNA Application
The miRNA purified by Serum/Plasma miRNA Isolation Kit can be used in various downstream molecular experiments, such as: RT-PCR, Real Time RT-PCR, Northern Blot, chip analysis, etc.
RNA storage
It is recommended to use RNase-Free ddH2O to elute RNA for immediate use in downstream experiments or store at -80℃. RNA can be stored for one year under -80℃ storage condition.
According to FOREGENE's Total Quality Management System, each batch of Serum/Plasma miRNA Isolation Kit is strictly tested for multiple times to ensure the reliability and stability of each batch of kit quality .
RNA extraction yield and purity
Using Serum/Plasma miRNA Isolation Kit can obtain the RNA from serum and plasma purification, and its yield is related to the sample itself, the initial amount of the sample, the freshness of the sample, the storage time of the sample, and the operation. The purified RNA OD260/280=1.8-2.1.
Work Flow
Contact Person: Maggie
Tel: +8615281067355