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Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.

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PCR Reaction Premix System 10ml PCR Hero PCR Kits With Dye Ultra Efficient

PCR Reaction Premix System 10ml PCR Hero PCR Kits With Dye Ultra Efficient

  • PCR Reaction Premix System 10ml PCR Hero PCR Kits With Dye Ultra Efficient
  • PCR Reaction Premix System 10ml PCR Hero PCR Kits With Dye Ultra Efficient
PCR Reaction Premix System 10ml PCR Hero PCR Kits With Dye Ultra Efficient
Product Details:
Place of Origin: China
Brand Name: FOREGNE
Certification: ISO CE
Model Number: PH-01015-B
Payment & Shipping Terms:
Minimum Order Quantity: 10 ml
Price: Negotiable
Packaging Details: Packing with safe trans carton
Delivery Time: 5-8 days
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100000 ml per month
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Detailed Product Description
Features: Higher Fidelity,Faster Amplification Speed Product Name: PCR Kits
Type: PCR Reaction Premix System MOQ: 10ml
OEM: Accept Application: For PCR Reaction
High Light:

10ml PCR Hero PCR Kits

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Ultra Efficient PCR Reaction Premix System

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PCR Reaction Premix System With Dye

PCR Hero (With Dye) Ultra-Efficient PCR Reaction Premix System PCR Kits Suitable For A Variety PCR Templates

 

 

Description:

 

This kit includes Foregene's unique new generation of Taq—Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

 

 

Specifications:

 

5ml, 10ml, 40ml

 

 

Kit(2× PCR HeroTM Mix) components:

 

0.1U Taq Hero/μl

400μM dNTPs

20mM Tris-HCl(pH8.8)

100mM KCl

5mM MgCl2

Other stabilizers and enhancers

DNA Loading Buffer

 

 

Features&advantages:

 

-Higher fidelity: 6 times that of ordinary Taq enzyme;

-Faster amplification speed : the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;

-More template adaptability: It can efficiently amplify various complex DNA templates with high GC content;

-Higher amplification efficiency: lower amplification cycles than ordinary Taq enzyme;

-Environmental tolerance is stronger: placed at 37°C for a week, maintaining more than 90% activity;

-It has 5’→3’ DNA polymerase activity and 5’→3’ exonuclease activity, without 3’→5’ exonuclease activity.

The kit is pre-mixed with Electrophoresis Loading Buffer, after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is simple and easy.

 

 

Practical examples

 

1.Reaction system 

2× PCR HeroTM Mix(dye) 10μl
Template DNA xμl
Primer 1(10μM) 0.4μl
Primer 2(10μM) 0.4μl
ddH2O Fill to 20μl
Total volume 20µl

 

2.Reaction conditions

Temperature Time Cycles
94°C 3min 1
94°C 10sec

 

 

25-40

55-65°C 10sec
72°C ≥10sec (5-20sec/kb)1*
72°C 5min 1

 

 

 

1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.

 

Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.

 

Kit application:

 

PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail

 

 

Storage and Shelf life:

 

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

 

Contact Details
Foregene Co., Ltd.

Contact Person: Foregene

Tel: +8613880676215

Send your inquiry directly to us
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