Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | China |
| Brand Name: | Foregene |
| Model Number: | PH-01013-B/01014-B/01015-B |
| Minimum Order Quantity: | 100kits |
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| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 3*1.7ml, 50ml |
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| Type: | PCR Mix | Applicable Samples: | PCR Amplification Of DNA Fragments,DNA Labeling,sequencing, PCR Plus A Tail |
| Product Name: | Fast Reagent PCR Hero(with Dye) PCR Kits Efficient For Lab Research Use | With Dye: | Yes |
| High Light: | PCR mix 3*1.7ml,PCR mix 50ml PH-01013-B,Fast reagent PCR Kits PH-01013-B |
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Fast reagent PCR Hero(with dye) PCR Kits efficient for lab research use
Product Pictures
Product Descprition
This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix (dye) system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
Taq Hero is a new generation of DNA polymerase modified by Foregene based on Taq enzyme.
Specifications:
PH-01013-B:2× PCR HeroTM Mix (dye) 1.7ml ×3
PH-01014-B:2× PCR HeroTM Mix (dye) 1.7ml ×6
PH-01015-B:2× PCR HeroTM Mix (dye) 1.7ml ×24
Product components:
2× PCR HeroTM Mix include:0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8), 100mM KCl, 5mM MgCl2,other stabilizers and enhancers,DNA Loading Buffer.
Features&advantages:
The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
Kit Application:
-PCR amplification of DNA fragments
-DNA labeling
-DNA sequencing
-PCR plus A tail
Practical examples:
1.Reaction System
| 2× PCR HeroTM Mix(dye) | 10μl |
| Template DNA | xμl |
| Primer 1(10μM) | 0.4μl |
| Primer 2(10μM) | 0.4μl |
| ddH2O | Fill to 20μl |
| Total volume |
20µl |
2.Reaction conditions
| Temperature | Time | Cycles |
| 94°C | 3min | 1 |
| 94°C | 10sec |
25-40 |
| 55-65°C | 10sec | |
| 72°C | ≥10sec (5-20sec/kb)1* | |
| 72°C | 5min | 1 |
1*: If using plasmid as template, the extension speed is 5sec/kb, we recommend using 10sec/kb; if using genomic DNA as the template, the extension speed is 10sec/kb, we recommend using 20sec/kb. If you use a highly complex template that is difficult to amplify, it is recommended to increase the extension time by 10sec/kb on the original basis.
Storage and Shelf life:
It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.
Contact Person: Foregene
Tel: +8613880676215
