First Strand CDNA Synthesis Reverse Transcription System RT Easy I Master Premix

Efficient and Highly-sensitive reverse transcription system RT Easy I Master Premix for first-strand cDNA synthesis

Description:

FOREGENE’s 2× RT Easy^TM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.

The RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT Easy^TM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.

This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.

RT Mix tolerance
 

After testing and analysis, 2×RT Easy^TM Mix shows extremely high tolerance to alcohol and guanidine salts. The RNA purified in the laboratory often has alcohol and guanidine salt residues, which have a strong inhibitory effect on reverse transcription, resulting in unsatisfactory reverse transcription effect or low reverse transcription efficiency. The tolerance of Foregene RT Easy series products to alcohol and guanidine salts makes reverse transcription easier and more efficient.

Alcohol Tolerance Analysis
 

Analysis of guanidine salt tolerance
 

Preparation before operation
 

It is strongly recommended that users read the instructions carefully before using this kit. The RT Easy series kits are simple, and fast to operate. The instructions provide the correct use of the entire kit. Please prepare necessary experimental materials and equipment before use.
 

Amount of reverse transcription primer
 

RNA template Amount
 

Best to use template RNA extracted from fresh samples or RNA stored at -80 ℃ (RNA should avoid repeated freezing and thawing).
RT Easy^TM I : (0.1pg-2.5μg total RNA or 0.01pg-0.5μg mRNA)/10μl reaction.
 

Safety

This product is for scientific research use only, please do not use it in medicine, clinical studies, food and cosmetics.
Wear suitable laboratory clothes, gloves, protective glasses, etc. when using chemicals.