T EasyTM I Master Premix for first-strand cDNA synthesis

Cat.No.RT-01011/01012

RT Easy I Master Premix For First-Strand CDNA Synthesis Fast And Highly Sensitive Reverse Transcription System

Description:

FOREGENE’s 2× RT EasyTM Mix can synthesize the first-strand cDNA at a temperature as high as 50°C, which is conducive to the reverse transcription reaction of complex secondary structure RNA templates.

The RNA purified in the laboratory often contains alcohol and guanidine salt residues, which have strong inhibitory properties on most reverse transcriptases, resulting in unsatisfactory reverse transcription effects or low reverse transcription efficiency. FOREGENE 2× RT EasyTM Mix shows extremely high tolerance to alcohol and guanidine salt, the highest tolerance to alcohol in RNA samples is 45%, and the highest tolerance to guanidine salt is 750mM. Even if the RNA is not pure, the reverse transcription reaction can be performed with FORREGENE 2×RT Mix. The unique reaction system makes the RT reaction easier, faster and more efficient, and the synthesis of the first strand cDNA can be completed in 25 minutes.

This product can be used in conjunction with Foregene's fluorescent quantitative product Real Time PCR EasyTM to obtain high-quality experimental results.

Specifications:

25T, 100T

Kit components:

Features&advantages:

u Efficient reverse transcription system, it only takes 25 minutes to complete the synthesis of the first strand cDNA.

u High-sensitivity reverse transcription system, pg-level templates can also get high-quality cDNA.

u The reverse transcription system has high thermal stability, the reaction temperature of the system can be as high as 50 ℃, and it has good reverse transcription performance.

u Even impure RNA samples (alcohol up to 45%, guanidine salt up to 750mM) can be subjected to reverse transcription reaction.

Kit component information

u 2× RT EasyTM Mix: Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, reaction buffer, optimizer and stabilizer, etc.

u Random Primer (50μM): 6 oligonucleotide random primers with a concentration of 50μM.

u Oligo(dT)18 Primer(50μM): a primer with 18 dT,the concentration is 50μM

Kit application

u Conventional RT-PCR

u Synthesis of cDNA for cloning and expression research

u Analysis of the primer extension method of the transcription initiation site

u RACE (rapid amplification of cDNA ends)

u Linear RNA amplification

u Chip mark

Precautions: (Please read the precautions carefully before using the kit)

u For templates, it is recommended to use RNA extracted from fresh samples or stored at -80℃ (RNA should avoid repeated freezing and thawing).

u In order to avoid RNase contamination, the experiment operation should be carried out in the RNase-Free space; the pipette tips and PCR centrifuge tubes used must be RNase-Free; and disposable gloves and masks should be worn.

u Before use, put the 2×RT EasyTM Mix on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and the specificity of PCR amplification.

u 2× RT EasyTM Mix does not add reverse transcription primers, you need to add appropriate concentrations of Random Primer or Oligo(dT)18 Primer or specific primers according to the experimental requirements.

Transport and storage conditions

1.Transportation conditions

The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

2. Storage conditions

RT EasyTM I is stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.