Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 |
| Place of Origin: | China |
| Brand Name: | FOREGENE |
| Certification: | ISO CE |
| Model Number: | RT-02132 |
| Minimum Order Quantity: | 1 kits |
|---|---|
| Price: | Negotiable |
| Packaging Details: | Packing with safe trans carton |
| Delivery Time: | 5-8 days |
| Payment Terms: | L/C, D/P, T/T |
| Supply Ability: | 100000 kits per month |
| Product Name: | Taqman RT QPCR Kit | Packing: | 200 Tests Per Kits |
|---|---|---|---|
| Application: | For RNA Reverse Transcription And PCR Amplification | OEM: | Accept |
| MOQ: | 1 Kits | Storage: | The Kit Should Be Stored At -20 ± 5℃ |
| High Light: | EasyTM RT qPCR Kit,RT qPCR Kit 1.7mL×3,Biology Applications taqman qpcr kit |
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EasyTM (One Step) Taqman RT QPCR Kit For Molecular Biology Applications
Intended use
The RT-qPCR EasyTM (One Step)-Taqman Kit is intended for molecular biology applications.
Introduction
The RT-qPCR EasyTM (One Step)-Taqman Kit provides rapid real-time quantification of RNA targets. The kit contains optimized components that allow both reverse transcription and PCR amplification to take place. With this kit, all steps can be performed in a single tube.
Kit contents
|
Cat. No. |
RT-02131 |
RT-02132 |
|
Number of reactions |
100 (20μL reaction) |
500 (20μL reaction) |
|
2× RT-qPCR EasyTM Mix-Taqman |
1mL |
1.7mL×3 |
|
20× ROX Reference Dye |
100μL |
0.5mL |
|
RNase-Free ddH2O |
1.7mL |
1.7mL×3 |
|
Protocol |
1 |
1 |
Shipping and storage conditions
When stored under these conditions and handled correctly, the kit can be kept one year without showing any reduction in performance.
The kit is shipped on ice pack or dry ice.
The kit should be stored at -20 ± 5℃.
Avoid repeated freeze-thaw cycles.
Protocol
Important notes before starting:
Set up all reactions on ice and mix the solutions completely before use.
This kit requires gene-specific primers for PCR.
An RNase-free environment should be maintained during reaction setup.
Procedure:
Prepare the PCR Reaction Mix
Combine the following components for the number of reactions required, plus 10% overage.
|
Component |
Volume/ reaction |
Final concentration |
|
2× RT-qPCR EasyTM Mix-Taqman |
10 μL |
1× |
|
Forward Primer (10 μM) |
0.8 μL |
50-900 nM 1* |
|
Reverse Primer (10 μM) |
0.8 μL |
50-900 nM 1* |
|
Probe (4 μM) |
1 μL |
200 nM |
|
Template (RNA) |
X μL |
0.1 pg-100 ng |
|
20× ROX Reference Dye |
- |
2* |
|
RNase-FreeddH2O |
(7.4-X) μL |
- |
|
Total Volume |
20 μL |
- |
1*: A final primer concentration of 400 nM is optimal for most reactions.
2*: Final ROX concentration for different instruments:
|
Instrument |
Dilution factor |
Amount per 20 μL reaction |
|
ABI PRISM 7000/7300/7700/7900HT/Step One |
1× |
1 μL |
|
ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P and Mx4000 |
0.5× |
0.5 μL |
Mix the components gently but thoroughly and dispense appropriate volumes into PCR tubes.
Run the reaction:
Thermal cycler conditions:
|
Step |
Temperature |
Time |
Cycles |
|
|
1 |
Reverse transcription |
42℃ |
10-30 min 1* |
1 |
|
2 |
Pre-denaturation |
94-95℃ |
5 min |
1 |
|
3 |
Denaturation |
94-95℃ |
10 sec |
30-45 |
|
Anneal/Extension |
60-65℃ |
20 sec 2* |
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or
|
Step |
Temperature |
Time |
Cycles |
|
|
1 |
Reverse transcription |
42℃ |
10-30 min 1* |
1 |
|
2 |
Pre-denaturation |
94-95℃ |
5 min |
1 |
|
3 |
Denaturation |
94-95℃ |
10 sec |
30-45 |
|
Anneal |
55-65℃ |
20 sec |
||
|
Extension |
72℃ |
20-30 sec 2* |
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1*: If satisfactory results are not obtained using 42°C, the reaction temperature may be increased up to 50°C.
2*: The extension rate of Foregene HotStar Taq DNA Polymerase is 2 kb/min in PCR.
After setting, save the file and run the reaction program.
Contact Person: Foregene
Tel: +8613880676215
