Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 
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| Place of Origin: | China |
| Brand Name: | FOREGENE |
| Certification: | ISO |
| Model Number: | DP-20034-B |
| Minimum Order Quantity: | 5 kits |
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| Price: | 88 usd per kit |
| Packaging Details: | Packing with safe trans carton |
| Delivery Time: | 7-10 working days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union |
| Supply Ability: | 10000 kits per month |
| Product Name: | PCR Easy Premix System | Application: | For PCR Reaction |
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| Packing: | 3x1.7 Ml Per Kit | MOQ: | 5 Kits |
| Leading Time: | 7-10 Working Days | Brand: | Foregene |
| Oem: | Accepted | ||
| High Light: | PCR Easy Premix System,PCR Easy With Dye Kit,PCR Reaction Premix System |
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This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
The product is pre-mixed with Electrophoresis Loading Buffer, and after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is more convenient and easier.
DP-20032-B:2× PCR EasyTM Mix(dye) 5ml(1.7ml × 3) DP-20033-B:2× PCR EasyTM Mix(dye) 10ml(1.7ml × 6) DP-20033-B:2× PCR EasyTM Mix(dye) 40ml(1.7ml × 24)
It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.
2× PCR EasyTM Mix(dye) include:0.1U Foregene Taq DNA Polymerase/μl,400 μM dNTPs,20 mM Tris-HCl(pH8.8),100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers,DNA Loading Buffer.
PCR amplification of DNA fragments
DNA labeling
DNA sequencing
PCR plus A tail
PCR EasyTM(With Dye) is convenient and quick to use, and can avoid the pollution during the PCR operation and the experimental error caused by multiple preparations of the reaction system to the greatest extent. Use only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2× PCR HeroTM Mix) of 2× PCR EasyTM(With Dye), add template and primers, and add deionized water to make up Volume, make the PCR Mix concentration in the reaction system 1×, then the reaction can be carried out.
1.Reaction System
| 2× PCR EasyTM Mix(dye) | 10μl |
| Template DNA | xμl |
| Primer 1(10μM) | 0.4μl |
| Primer 2(10μM) | 0.4μl |
| ddH2O | Fill to 20μl |
| Total volume | 20µl |
2.Reaction conditions
| Temperature | Time | Cycles |
| 94°C | 3min | 1 |
| 94°C | 10sec |
25-40 |
| 55°C | 20sec | |
| 72°C | 2kb/min | |
| 72°C | 5min | 1 |
Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.
Contact Person: David
Tel: +8616602829025
