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Foregene Co., Ltd.

Foregene Co., Ltd.

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PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research

PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research

PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research
PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research
PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research
Product Details:
Place of Origin: China
Brand Name: FOREGENE
Certification: ISO
Model Number: DP-20034-B
Payment & Shipping Terms:
Minimum Order Quantity: 5 kits
Price: 88 usd per kit
Packaging Details: Packing with safe trans carton
Delivery Time: 7-10 working days
Payment Terms: L/C, D/A, D/P, T/T, Western Union
Supply Ability: 10000 kits per month
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Detailed Product Description
Product Name: PCR Easy Premix System Application: For PCR Reaction
Packing: 3x1.7 Ml Per Kit MOQ: 5 Kits
Leading Time: 7-10 Working Days Brand: Foregene
Oem: Accepted
High Light:

PCR Easy Premix System

,

PCR Easy With Dye Kit

,

PCR Reaction Premix System

PCR Easy (With Dye) Kit Efficient PCR reaction premix system For Molecular bio research use

Product Introduction

This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.

The product is pre-mixed with Electrophoresis Loading Buffer, and after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is more convenient and easier.

PCR Easy (With Dye) Kit Efficient PCR Reaction Premix System For Molecular Bio Research 0

Kit Series

DP-20032-B:2× PCR EasyTM Mix(dye) 5ml(1.7ml × 3) DP-20033-B:2× PCR EasyTM Mix(dye) 10ml(1.7ml × 6) DP-20033-B:2× PCR EasyTM Mix(dye) 40ml(1.7ml × 24)

 

Storage Conditions

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.

 

Kit Contents

2× PCR EasyTM Mix(dye) include:0.1U Foregene Taq DNA Polymerase/μl,400 μM dNTPs,20 mM Tris-HCl(pH8.8),100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers,DNA Loading Buffer.

 

Kit application

PCR amplification of DNA fragments

 

DNA labeling

 

DNA sequencing

 

PCR plus A tail

 

Instructions

PCR EasyTM(With Dye) is convenient and quick to use, and can avoid the pollution during the PCR operation and the experimental error caused by multiple preparations of the reaction system to the greatest extent. Use only half the volume of the reaction system (for example, if the reaction system is 20μl, take 10μl 2× PCR HeroTM Mix) of 2× PCR EasyTM(With Dye), add template and primers, and add deionized water to make up Volume, make the PCR Mix concentration in the reaction system 1×, then the reaction can be carried out.

Practical examples

1.Reaction System

2× PCR EasyTM Mix(dye) 10μl
Template DNA xμl
Primer 1(10μM) 0.4μl
Primer 2(10μM) 0.4μl
ddH2O Fill to 20μl
Total volume 20µl

2.Reaction conditions

Temperature Time Cycles
94°C 3min 1
94°C 10sec

 

25-40

55°C 20sec
72°C 2kb/min
72°C 5min 1

 

Note: For 10µl and 20µl systems, if the PCR machine does not have a thermal cover, add an equal volume of mineral oil. The PCR reaction conditions are based on the specific conditions of the template, primer set, and the GC content and length of the primers to design the best reaction conditions, including annealing temperature, extension time, etc.

Contact Details
Foregene Co., Ltd.

Contact Person: David

Tel: +8616602829025

Send your inquiry directly to us
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