Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 |
| Place of Origin: | China |
| Brand Name: | Foregene |
| Model Number: | RT-02131/02132 |
| Minimum Order Quantity: | 100kits |
|---|---|
| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Specifications: | 100T (20μl System),500 T(20μl System) |
|---|---|---|---|
| Product Name: | Quick And Efficient RT-qPCR Easy (One Step)-Taqman QPCR Kit For Lab Use | Other Name: | One-step Real-Time RT-PCR Master Mix |
| High Light: | 20ul system one step qPCR,one step qPCR 100Rxns,20ul system kit qPCR |
||
Quick and efficient RT-qPCR EasyTM (One Step)-Taqman qPCR Kit for lab use
Product Descprition
Foregene One-Step RT-PCR Easy series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, thus the experimental steps simplified, the experimental plan optimized, and the experimental efficiency improved.
RT-qPCR EasyTM (One Step)-Taqman using Foregene HotStar Taq DNA Polymerase, the optimized Taqman qPCR system can quickly and specifically perform Real Time RT-qPCR quantitative detection of trace RNA templates.
Product components:
|
RT-qPCR EasyTM (One Step)-Taqman |
||
|
Kit components |
RT-02131 | RT-02132 |
| 100Rxns (20μl system) | 500 Rxns (20μl system) | |
| 2× RT-qPCR EasyTM Mix-Taqman | 1ml | 1.7ml×3 |
| 20× ROX Reference Dye | 100μl | 0.5ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Instruction For Use | 1 | 1 |
Features&advantages:
Product quality control:
In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-qPCR Easy (One Step)-Taqman qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Form 1:RT-qPCR system preparation
| RT-qPCR system contents | Amount | Final concentration |
| 2× RT-qPCR EasyTM Mix-Taqman | 10μl | 1× |
| Forward Primer (10μM) | 0.8μl | 50-900nM |
| Reverse Primer (10μM) | 0.8μl | 50-900nM |
| Probe(4μM) | 1μl | 200nM |
| Template(RNA) | Xμl | 0.1pg-100ng |
| 20× ROX Reference Dye | - |
1* |
| RNase-FreeddH2O | (7.4-X)μl | |
| Total Volume | 20μl |
Note: Forward Primer and Reverse Primer are specific primers for the target gene. The qPCR system can be adjusted according to experimental needs and PCR model. For the final concentration of most primers, we recommend 400nM. Please adjust the dosage of the specific primer and Probe according to the prepared concentration according to our recommended final concentration. For qPCR with 50μl system, please refer to the 20μl system to adjust the amount of reagents proportionally.
1*:Choose the appropriate final concentration of ROX Reference Dye according to different quantitative PCR instruments. The optimal concentration of ROX Reference Dye for common quantitative PCR machines is shown in the following table:
| Quantitative PCR machine | ROX Reference Dye final concentration |
|
ABI PRISM 7000/7300/7700/7900HT/Step One ,etc. |
1× ( eg. 20μl system,add 1μl 20×ROX Reference Dye) |
| ABI 7500,7500 Fast,Stratagene Mx3000P,Mx3005P and Mx4000 etc. | 0.5× (eg. 20μl system , add 0.5μl 20×ROX Reference Dye) |
|
Roche PCR machine,Bio-Rad PCR machine,Eppendorf quantitative PCR machine,etc. |
No need to add ROX Reference Dye |
Storage and Shelf life:
The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.
Contact Person: Foregene
Tel: +8613880676215
