Quick and efficient RT-qPCR Easy (One Step)-SYBR Green I qPCR Kit lab use

Product Descprition

RT-qPCR Easy^TM (One Step)-SYBR Green I uses a unique system to effectively combine RT and Real Time PCR reactions, which can quickly complete the quantitative detection of genes. The product has strong tolerance, high specificity and stability. The product contains the company's unique Foregene HotStar Taq DNA Polymerase, which has the advantages of faster amplification (2Kb/min), high amplification efficiency, strong specific amplification ability, and low mismatch rate compared with ordinary Taq enzymes. It is used here for Real Time RT-PCR to reduce non-specific amplification and improve the accuracy of PCR.

Product components:

Features&advantages:

• One-step kit makes reverse transcription and qPCR two reactions in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH₂O.
• The kit can quickly and efficiently quantitatively analyze viral RNA or trace RNA.
• The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
• The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
• RT-qPCR Easy^TM (One Step)-SYBR Green I kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for customers to use in different models of quantitative PCR instruments.

Kit component information

• 2× RT-qPCR Easy^TM Mix-SYBR:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase, optimized ratio of dNTPs, SYBR GreenI, Mg²⁺, stabilizers, enhancers, and optimizers.
• OX Reference Dye:it is generally used on Real Time PCR amplifiers of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. The concentration of ROX Reference Dye required by different instruments is different, and the user can add it according to the recommended concentration of the instrument.

Product quality control:

In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-qPCR Easy (One Step)-SYBR Green I qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.

Form 1:RT-qPCR system preparation

Storage and Shelf life:

The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.