Foregene Co., Ltd.
Foregene Co., Ltd.

Foregene Co., Ltd.


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Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use

Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use

  • Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use
  • Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use
  • Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use
Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use
Product Details:
Place of Origin: China
Brand Name: Foregene
Model Number: RT-02111/02112
Payment & Shipping Terms:
Minimum Order Quantity: 100kits
Price: Negotiable
Packaging Details: craft paper bag
Delivery Time: 5-8 working days
Payment Terms: L/C, D/A, D/P, T/T
Supply Ability: 100000kits per month
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Detailed Product Description
Factory: Foregene Specifications: 100T (20μl System),500 T(20μl System)
Product Name: Quick And Efficient RT-qPCR Easy (One Step)-SYBR Green I QPCR Kit Lab Use Other Name: One-step Real-Time RT-PCR Master Mix
High Light:

Foregene one step qPCR kit


SYBR green qPCR kit 500T


one step qPCR kit 1.7ml×3


Quick and efficient RT-qPCR Easy (One Step)-SYBR Green I qPCR Kit lab use



Product Descprition

Foregene One Step SYBR Green QPCR Kit 1.7ml×3 Lab Use 0


RT-qPCR EasyTM (One Step)-SYBR Green I uses a unique system to effectively combine RT and Real Time PCR reactions, which can quickly complete the quantitative detection of genes. The product has strong tolerance, high specificity and stability. The product contains the company's unique Foregene HotStar Taq DNA Polymerase, which has the advantages of faster amplification (2Kb/min), high amplification efficiency, strong specific amplification ability, and low mismatch rate compared with ordinary Taq enzymes. It is used here for Real Time RT-PCR to reduce non-specific amplification and improve the accuracy of PCR.


Product components:


RT-qPCR EasyTM (One Step)-SYBR Green I
Kit Contents RT-02111 RT-02112
100T (20μl system) 500T (20μl system)
2× RT-qPCR EasyTM Mix-SYBR 1ml 1.7ml×3
50× ROX Reference Dye 200μl 1ml
RNase-Free ddH2O 1.7ml 1.7ml×3
Instruction Manual 1piece 1 piece



  • One-step kit makes reverse transcription and qPCR two reactions in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH2O.
  • The kit can quickly and efficiently quantitatively analyze viral RNA or trace RNA.
  • The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.
  • The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.
  • RT-qPCR EasyTM (One Step)-SYBR Green I kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for customers to use in different models of quantitative PCR instruments.

Kit component information

  • 2× RT-qPCR EasyTM Mix-SYBR:Foregene Reverse Transcriptase, RNase Inhibitor, Foregene HotStar Taq DNA Polymerase, optimized ratio of dNTPs, SYBR GreenI, Mg2+, stabilizers, enhancers, and optimizers.
  • OX Reference Dye:it is generally used on Real Time PCR amplifiers of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. The concentration of ROX Reference Dye required by different instruments is different, and the user can add it according to the recommended concentration of the instrument.


Product quality control:


In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of RT-qPCR Easy (One Step)-SYBR Green I qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.



Form 1:RT-qPCR system preparation


RT-qPCR system additions Amount Final concentration
2× RT-qPCR EasyTM Mix-SYBR 10μl
Forward Primer (10μM) 0.5μl 0.2-0.25μM 1*
Reverse Primer (10μM) 0.5μl 0.2-0.25μM 1*
Template(RNA) Xμl 0.1pg-100ng
50× ROX Reference Dye - 2*
RNase-FreeddH2O (9-X)μl  
Total Volume 20μl  


1*: Usually the final concentration of primer is 0.2-0.25μM to get better results. When the reaction performance is poor, the primer concentration can be adjusted within the range of 0.1-0.5μM.

Note: Forward Primer and Reverse Primer are specific primers for the target gene.

2*: Choose the appropriate final concentration of ROX Reference Dye according to different quantitative PCR instruments. The optimal concentration of ROX Reference Dye for common quantitative PCR machines is shown in the following table:


Quantitative PCR instrument ROX Reference Dye final concentration
ABI PRISM 7000/7300/7700/7900HT/Step One etc. 5× (i.e.20μl system, add 2μl 50×ROX Reference Dye)
ABI 7500,7500 Fast, Stratagene Mx3000P, Mx3005P, and Mx4000, etc. 1× (i.e.20μl system, add 0.4μl 50×ROX Reference Dye)
Roche PCR instrument, Bio-Rad PCR instrument, Eppendorf quantitative PCR instrument, etc. No need to add ROX Reference Dye


Storage and Shelf life:


The kit should be stored at -20°C. Store the product in a constant temperature refrigerator at -20°C immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.


Contact Details
Foregene Co., Ltd.

Contact Person: Foregene

Tel: +8613880676215

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Foregene Co., Ltd.
Building D2, block A, Tri-medical innovation center, No.18, North Bayi Road, Yongning Town, Wenjiang District, Chengdu City, Sichuan Province, P.R.China
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