Laboratory 99.9% One Step RT PCR Taqman RT QPCR Kit RT-02132

Laboratory One-step RT-PCR Master Mix Reagent RT-qPCR Easyᵀᴹ (One Step)-Taqman

Description:

Foregene One-Step RT-PCR Easy series products realize the integrated reaction from RNA to double-stranded DNA, that is, reverse transcription and PCR amplification are completed in the same reaction centrifuge tube and the same reaction system, thus the experimental steps simplified, the experimental plan optimized, and the experimental efficiency improved.

RT-qPCR Easy^TM (One Step)-Taqman using Foregene HotStar Taq DNA Polymerase, the optimized Taqman qPCR system can quickly and specifically perform Real Time RT-qPCR quantitative detection of trace RNA templates.

 
 
Specifications:

100T, 500T
 

Kit components:

 
 
Features&advantages:

- One-step kit enables reverse transcription and PCR to be carried out in the same tube, only need to add template RNA, specific PCR primers and RNase-Free ddH₂O.

- Real-time quantitative analysis of viral RNA or trace RNA can be carried out quickly and accurately.

- The kit uses a unique Foregene reverse transcription reagent and Foregene HotStar Taq DNA Polymerase combined with a unique reaction system to effectively improve the amplification efficiency and specificity of the reaction.

- The optimized reaction system makes the reaction have higher detection sensitivity, stronger thermal stability, and better tolerance.

- RT-qPCR Easy^TM (One Step)-Taqman kit comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for end users to use in different models of quantitative PCR instruments.

Kit application:

- This kit is used for rapid and accurate detection of trace RNAs such as RNA viruses.

- It is especially suitable for rapid detection of RNA templates with high GC content or complex secondary structure.

Work Flow:

Transportation conditions:

The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.

Storage conditions:

The kit is stored at -20℃. Store the product in a constant temperature refrigerator at -20℃ immediately after receipt. If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

Notice:

♦ Reagents should avoid repeated freezing and thawing, otherwise the performance of the reagents will decrease or become invalid.

♦ The reagents in the kit should be protected from light and stored at -20°C.

♦ It is recommended to use fresh sample extraction or template RNA stored at -80℃ (RNA should avoid repeated freezing and thawing).

♦ In order to avoid RNase contamination, the experiment operation should be performed in the RNase-Free space; the pipette tips and PCR tubes used must be RNase-Free; and disposable gloves and masks should be worn.

♦ This kit must be used with specific primers for experiments. Please select the specific primers for the gene to be amplified according to the needs of the experiment.

♦ Before use, put 2× RT-PCR EasyTM Mix on ice to completely melt, flick and mix well before use; the preparation of the system should be operated on an ice bath to improve the performance of the kit and the specificity of PCR amplification .