COA 99.9% qPCR Kits SYBR Green I High Amplification Efficiency Sensitivity

High amplification efficiency and sensitivity qPCR Kits by SYBR Green I

Description

The 2× Real PCR Easy^TM Mix-SYBR provided by the kit is a new premix system that uses SYBR Green I for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity. At the same time, ROX is provided as an internal control dye. The fluorescence intensity of this kit is 3-5 times that of similar products, and can more sensitively and intuitively reflect the concentration of target template DNA.

2× Real PCR Easy^TM Mix-SYBR contains our company’s unique hot-start Foregene Taq DNA Polymerase. Compared with ordinary Taq enzymes, this enzyme has the advantages of high amplification efficiency, strong specific amplification ability, and low mismatch rate. Used for fluorescent quantitative PCR reaction can reduce non-specific amplification and improve the accuracy of PCR.

Kit components

Features&advantages

- Simple—2× PCR Mix to reduce experimental error and operation time

- Specific—optimized buffer and hot-start Taq enzyme can prevent non-specific amplification and primer dimer formation

- High sensitivity—can detect low copies of template

- Good versatility—compatible with most real-time quantitative PCR instruments

Kit application

Fluorescence quantitative PCR performs template quantitative analysis/conventional PCR amplification.

Work Flow

Graphic

Transport conditions

The whole process is transported in a low-temperature ice box to ensure that the kit is in a state of <4℃.

Storage conditions

Store at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days). If the storage conditions are appropriate, the product will not degrade any performance during the 1-year validity period.

Notice

- 2× Real PCR EasyTM Mix-SYBR is stored at -20℃. Repeated freezing and thawing should be avoided, otherwise the PCR efficiency will be affected.

- When using, please turn the 2× Real PCR EasyTM Mix-SYBR upside down and mix gently to avoid foaming, and centrifuge briefly before use. If the reagents are not evenly mixed, the reaction performance will decrease. Do not use a shaker to mix well.

- 2× Real PCR EasyTM Mix-SYBR contains SYBR Green I. Avoid strong light when storing or preparing PCR reaction solution.

Please use new (non-contaminated) pipette tips, PCR tubes, etc. for the preparation and aliquoting of the reaction solution to avoid contamination as much as possible.