RNA Extraction process shortened from 1h to 11min -- what have we experienced?

About RNA extraction

Thirty years ago, for RNA extraction, what we could think was only how to obtain complete RNA, however, there was no requirement for extraction speed (The first-generation Techology the Trizol method born). With the iterative update of RNA extraction technology, what we can do is not limited to obtaining results. When faced with the extraction of the first-generation Trizol-based reagents, experimenters were generally troubled by its cumbersome operation steps; although after many optimizations and innovations, it still took a lot of time. At the same time, organic reagents such as phenol and chloroform need to be used in the extraction process, which also threatens the safety of relevant personnel to a certain extent.

Efficient, convenient and safe

Finding an efficient, convenient and safe RNA extraction product has become the primary goal of experimenters. Therefore, The Silica Spin Column adsorption method for RNA extraction came into being. Foregene's third-generation RNA extraction products draw on the advantages of the previous generation column extraction products, using(DNA-Cleaning + RNA only) two Spin Column technology to remove gDNA and tissue debris at the same time, without DNase treatment of residual gDNA, and no longer use phenol, etc. Toxic and harmful reagents, so as to achieve short time, high yield, good purity, and low risk of RNA degradation.

Operation process comparison chart

Experimental results comparison chart

Foregene total RNA isolation kit has high RNA extraction yield, good purity, and the extraction effect of miRNA is better than other brand kits

Electrophoresis of 50 mg of mouse liver total RNA extracted by different methods

The first generation (1: Trizol)

Second generation (2: RNA extraction kit from company A)

The third generation (3: RNA extraction kit plus from company A, 4: Foregene:RNA Isolation kits)

qPCR graph (amplification curve graph)

Genomic DNA must be effectively removed during RNA extraction, otherwise it will have uncontrollable effects on downstream experiments (early CT value, false positives). Foregene next-generation kit can perfectly remove genomic DNA without the addition of DNase, saving a lot of time, while avoiding degradation caused by exogenous RNase contamination.

The first generation (green indicates TRlzol-, red indicates TRlzol+)

Second-generation (Blue indicates Company A RNA extraction kit -, red indicates Company A RNA extraction kit +)

The third generation (blue indicates company A RNA extraction kit plus, green indicates Foregene RNA Isolation kits)

(Note: "_" in the qPCR graph indicates that DNase has not been added, and the contamination of DNA has not been removed; "+" indicates that DNase has been added, and the contamination of DNA has been removed)

Complete RNA extraction within 11min

No need for years of nucleic acid extraction experience accumulation, Foregene high-efficiency total RNA isolation kits series products can allow you to obtain high-yield and high-purity RNA within 11 minutes to meet various downstream experimental needs.

Up to now, Foregene total RNA isolaton kits series products have been recognized by a large number of customers and have contributed to many high-scoring literatures. The customer's actual use of the feedback electrophoresis chart and the quantitative PCR amplification curve after reverse transcription show that the RNA extracted by the use of Foregene products is not only high in concentration, but also in good integrity. Only the product technology that has been tested by the market can be favored by everyone.

Cell Total RNA Isolation Kit

High RNA yield

Fast: Finish RNA Isolation in 11 minutes

Safety: None Organic chemical added

Some high-scoring citations:

1. Yuan Fang, Zezhong Liu, Yang Qiu, et al. Inhibition of viral suppressor of RNAi proteins by designer peptides protects from enteroviral infection in vivo, Immunity, 54(10), 2021: 2231-2244.e6. (IF:31.745, Viral RNA Isolation Kit，Cat No.RE-02011)

https://www.sciencedirect.com/science/article/pii/S1074761321003617

2. Ren, Y., Wang, A., Wu, D. et al. Dual inhibition of innate immunity and apoptosis by human cytomegalovirus protein UL37x1 enables efficient virus replication. Nat Microbiol 7, 1041–1053 (2022). (IF:30.964, Cell Total RNA Isolation Kit，Cat No.RE-03111)

https://doi.org/10.1038/s41564-022-01136-6