Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
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| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | COA |
| Model Number: | 50T,200T |
| Minimum Order Quantity: | 1 BOX |
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| Price: | USD200/BOX |
| Delivery Time: | 1-7 days |
| Payment Terms: | T/T, L/C, Western Union |
| Supply Ability: | 10000 BOX per Month |
| Product Name: | Cell Total RNA Isolation Kit | Specification: | 50 Preps, 200 Preps |
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| CAS No.: | RE-03111, RE-03114 | Usage: | Cell Total RNA Purification |
| Classification: | General Reagents | Purity: | 99.9% |
| Shelf Life: | 1-2 Years | ||
| High Light: | 50 Preps Cell Total RNA Isolation Kit,200 Preps Cell Total RNA Isolation Kits |
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Spin Column Cell Total RNA Isolation Kits 50 Preps 200 Preps no need ice bath
Description:
This kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from cells cultured in 96, 24, 12, and 6-well plates.
The kit provides an efficient DNA-Cleaning Column, which can easily separate the supernatant and cell lysate, bind and remove genomic DNA. The operation is simple and time-saving.
The RNA-only Column can efficiently bind RNA with a unique formula. A large number of samples can be processed simultaneously.
Specifications:
50 Preps, 200 Preps
Kit components:
| Kit composition | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Buffer cRL1* | 25 mL | 100 mL |
| Buffer cRL2 | 15 mL | 60 mL |
| Buffer RW1* | 25 mL | 100 mL |
| Buffer RW2 | 24 mL | 96 mL |
| RNase-Free ddH2O | 10 mL | 40 mL |
| RNA-Only Column | 50 | 200 |
| DNA-Cleaning Column | 50 | 200 |
| Instruction | 1 | 1 |
*Please wear gloves and take protective measures during the operation as Buffer cRL1 and Buffer RW1 contain irritating chaotropic salts.
Product Information
| Format | Spin column | Purification component | Foregene column, reagent |
| Flux | 1-24 samples | Time per prep | ~11 min (24 samples) |
| Centrifuge | Desk centrifuge | Pyrolysis separation | Centrifugal separation |
| Sample | Cultured cell | Samples amount | 104 -106 |
| Elution volume | 20-100 μL | Maximum loading volume | 850 μL |
Features&advantages:
- The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.
- The whole kit is RNase-Free, no need to worry about RNA degradation.
- DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
- High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
- Fast speed: easy to operate and can be completed in 11 minutes.
- Safety: No organic reagent is required.
- High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.
Kit application:
It is suitable for extraction and purification of total RNA from cultured cells in 96, 24, 12, and 6-well plates.
Work Flow:
Diagram:
The agarose gel battery diagram of Cell Total RNA Isolation Kit treated the above different numbers of cells,20μl volume elution, take 2μl purified total RNA 1%.
Storage and Shelf life:
The kit can be stored for 12 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time(24 months).
Buffer cRL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol(optional).
Kit component information
Buffer cRL1: Provides the environment required for cell lysis.
Buffer cRL2: Provides a specific upper column environment for RNA.
Buffer RW1: Removes impurities such as proteins and DNA from RNA.
Buffer RW2: Remove residual salt ions from RNA.
RNase-Free ddH2O: Total RNA on elution of the purified column membrane.
DNA-Cleaning Column: Specifically adsorbs DNA in cell lysates and filters to remove solid impurities from the lysates.
RNA-only Column: Total RNA specifically adsorbed through the DNA-Cleaning Column filtrate.
Notice:
- All procedures are carried out at room temperature (15-25℃) , including centrifugation. Do not use ice bath or centrufuge at low-temperature (4℃) .
- The sample should avoid repeated freezing and thawing, otherwise it will lead to the degradation of the extracted RNA and the yield of RNA will also decrease.
- The yield and quality of RNA is tightly related to sample size and volume of elution. For every 500μL of buffer cRL1, the recommended maximum cell volume is 106.
- Before using the kit, please add 2-hydroxy-1-ethanethiol to Buffer cRL1 (10μL 2-hydroxy-1-ethanethiol per 1mL Buffer cRL1). If the extracted RNA is not used to clone full-length cDNA, but only used for other downstream operations such as qPCR or sequencing analysis, it is not necessary to add 2-hydroxy-1-ethanethiol, and the result will not be affected.
- Before using the kit, please add anhydrous ethanol to buffer cRL2 and Buffer RW2 referring to the label on the reagent bottle for the dosage.
- The volume of elution should be no less than 20μL, otherwise it will influence RNA yield.
- Please check if there is any precipitate in the Buffer cRL1 and Buffer RW1. If the precipitate can be seen after storage at low temperature, the solution should be placed at room temperature(15-25℃) or 37℃ for period of time. Use the solution after dissolving and blending.
Contact Person: Maggie
Tel: +8615281067355
