Foregene Enpower | Application of Cell Direct RT-qPCR Kit in Extracellular Vesicles

Background

In recent years, extracellular vesicles (EVs) have attracted people's attention as a potential therapeutic tool; however, the therapeutic effect of EVs on endometriosis has not been reported. Endometriosis is a common non-malignant gynecological disease that affects 10-15% of women of childbearing age, causing a variety of symptoms, resulting in a decline in the quality of life and a huge social burden.

Article Introduction

On July 20, 2021, the research group of Professor Wang Guoyun from Qilu Hospital of Shandong University published a research paper entitled "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis" on Frontiers in immunology, which discussed the derivation of M1 macrophages. The feasibility of nanovesicles (NVs) in the treatment of endometriosis.

This article uses continuous extrusion method to prepare M1NVs, and uses co-culture method to study the changes in angiogenesis, migration, invasion and other indicators of eutopic endometrial stromal cells (EM-ESCs) from patients with endometriosis. At the same time, a mouse model of endometriosis was established, and the mice were treated with PBS, MONVs or M1NVs, respectively, to evaluate the efficacy and safety of M1NV in the treatment of endometriosis.

The results showed that in vitro M1NVs can directly or indirectly inhibit the migration and invasion of EM-ESCs, and inhibit angiogenesis. In the mouse model, M1NVs inhibit the occurrence of endometriosis through M2 macrophage reprogramming without causing organ damage. It shows that M1NVs can directly inhibit the occurrence of endometriosis, and can also be inhibited by repolarizing M2 type macrophages to M1 type. Therefore, the use of M1NVs may be a new method for the treatment of endometriosis.

Foregene Help

In the study, because M1NV was prepared by continuously squeezing M1 macrophages, the article used qRT-PCR to detect the pro-inflammatory factors and M1 macrophage markers iNOS, TNF-a and IL-6 mRNA in M1NV and M1 macrophages. Relative multiples of change. The results showed that M1NVs contained more pro-inflammatory factor mRNA and M1 macrophage markers, indicating that M1NVs can effectively retain the functional characteristics of M1 cells. This research method uses the QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I of Chengdu Forgene

Cell Direct RT-qPCR Kit details

Application scenarios:

1. Gene regulation and expression analysis, verification of gene overexpression or interference effect, drug screening, etc.;

2. Gene expression detection of difficult-to-cultivable cells such as primary cells, stem cells, and nerve cells;

3. Detection of mRNA in samples such as exosomes and nanovesicles.

Features: