Place of Origin: | China |
Brand Name: | Foregene |
Model Number: | DRT-01011/DRT-01012 |
Minimum Order Quantity: | 100kits |
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Price: | Negotiable |
Packaging Details: | craft paper bag |
Delivery Time: | 5-8 working days |
Payment Terms: | L/C, D/A, D/P, T/T |
Supply Ability: | 100000kits per month |
Factory: | Foregene | Specifications: | 200T, 1000T |
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Type: | Cell Direct RT-qPCR | Application: | Cultured Cells |
Product Name: | Quick And Easy One-step Cell Direct RT QPCR Kit SYBR GREEN I And Taqman | Key Word: | Direct RT-qPCR |
High Light: | Buffer CL RT qPCR Kit,RT qPCR Kit 200T 1000T,1000T Cultured Cell Direct RT-qPCR |
Quick and easy one-step Cell Direct RT qPCR Kit SYBR GREEN I and Taqman
Product Descprition
This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.
5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
Product components:
Part I | Buffer CL |
Foregene Protease Plus II | |
Buffer ST | |
Part II | DNA Eraser |
5× Direct RT Mix | |
2× Direct qPCR Mix-SYBR | |
50× ROX Reference Dye | |
RNase-Free ddH2O | |
Instructions |
Features&advantages:
Kit parameters:
Application:
-Scope of application: cultured cells.
-RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.
-The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.
Product quality control:
In accordance with FOREGENE’s Total Quality Management System (FOREGENE’s Total Quality Management System), each batch of Cell Direct RT-qPCR kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
Storage and Shelf life:
Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.
Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.
Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
Contact Person: Foregene
Tel: +8613880676215